摘要
目的:探究古草生机汤对H22荷瘤小鼠体内实体瘤的抑瘤功效和对机体的免疫调节作用机制。方法:本研究采用H22实体瘤动物模型,随机分为空白组、模型组、阳性药组、古草生机汤低、高剂量组,灌胃给药7 d,计算各组小鼠的体质量增长率、肿瘤抑制率、胸腺及脾指数,小鼠实体瘤病理切片苏木精-伊红(HE)染色观察瘤体组织和细胞形态,酶联免疫吸附试验法检测小鼠血清中γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)、Fas、Fas配体、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的含量,实时荧光定量PCR测量小鼠瘤组织中Bax和Bcl-2mRNA的相对表达量。结果:古草生机汤有促进H22荷瘤小鼠体质量增长、抑制体内实体瘤增长和促进肿瘤凋亡坏死的作用,可提高小鼠的胸腺指数同时降低脾脏指数,可显著提高小鼠血清中IFN-γ、TNF-α、IL-2的含量并降低Fas、Fas配体、AST、ALT的含量(均P<0.01),能够上调小鼠瘤组织中Bax mRNA的表达并下调Bcl-2mRNA的表达。结论:古草生机汤具有明显的体内抗肝肿瘤药效,在抑制肿瘤生长、促进肿瘤细胞凋亡、调节机体免疫方面有一定作用。
Objective:To explore the anti-tumor effects of Gucao Shengji Decoction on H22 tumor-bearing mice and its immunomodulatory mechanism on the body.Methods:In this study,an H22 tumor-bearing model was established in mice.Mice were randomly divided into a blank group,a model group,a positive drug group,and low-and high-dose Gucao Shengji Decoction groups.The mice were orally administered the corresponding drugs for 7 days.The body weight growth rate,tumor inhibition rate,thymus index,and spleen index of the mice in each group were calculated.The tumor tissues and cell morphology were observed by hematoxylin-eosin(HE)staining.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of gamma interferon(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-2(IL-2),Fas,Fas ligand,aspartate aminotransferase(AST),and alanine aminotransferase(ALT)in the mouse serum.Real-time fluorescence-based quantitative PCR was used to measure the relative expression levels of Bax and Bcl-2 mRNA in the tumor tissues of the mice.Results:Gucao Shengji Decoction promoted the growth of H22 tumor-bearing mice,inhibited the growth of solid tumors,and induced tumor apoptosis and necrosis.Additionally,it increased the thymus index,decreased the spleen index of the mice,significantly increased the levels of IFN-γ,TNF-α,and IL-2 in the mouse serum,decreased the levels of Fas,Fas ligand,AST,and ALT(all P<0.01),upregulated the expression of Bax mRNA,and downregulated the expression of Bcl-2 mRNA in the tumor tissues.Conclusion:Gucao Shengji Decoction has significant anti-liver tumor effects in vivo,which can inhibit tumor growth,promote tumor cell apoptosis,and modulate the body′s immune response.
作者
张伊冰
亓焕伟
李家峰
亓焕明
李瀛伟
包永睿
孟宪生
ZHANG Yibing;QI Huanwei;LI Jiafeng;QI Huanming;LI Yingwei;BAO Yongrui;MENG Xiansheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Shenyang Baicaojishi Oncology Medical&Drug Research Center,Shenyang 110041,China;Engineering Laboratory of Modern Chinese Medicine Research of Liaoning Province,Dalian 116600,China;Technical Innovation Center of Multidimensional Analysis of Traditional Chinese Medicine of Liaoning Province,Dalian 116600,China)
出处
《世界中医药》
CAS
2023年第14期1979-1985,共7页
World Chinese Medicine
基金
2016、2017年度辽宁省创新团队研究项目(LT2017015)——中药药效物质组学及作用机制整合研究。
关键词
古草生机汤
H22荷瘤小鼠
实体瘤
肝癌
抗肿瘤
抑制
肿瘤生长
促肿瘤细胞凋亡
免疫调节
Gucao Shengji Decoction
H22 tumor-bearing mice
Solid tumor
Hepatocellular carcinoma
Anti-tumor
Inhibiting
Tumor growth
Promoting tumor cell apoptosis
Immunomodulation
