摘要
孕期雌鼠(GD0至GD20)经水暴露于质量浓度为0、0.1、1.0、10.0 mg·L^(-1)的As^(3+)中.其中高剂量组于GD15-GD19时进行硒干预.GD20时取血液和肝脏,并计算肝脏系数.电感耦合等离子体质谱检测血清和肝脏中的元素含量;液相色谱串联三重四极杆质谱进行拟靶向脂质组学检测;全自动生化分析仪检测血清生化指标.结果显示,高剂量砷暴露可诱发大鼠脂代谢紊乱,硒干预后会改变血清中脂质代谢过程.
Pregnant female rats(GD0 to GD20) were exposed to As^(3+) in water at concentrations of 0,0.1,1.0,and 10.0 mg·L^(-1).The high-dose group underwent selenium intervention at GD15-GD19.Blood and liver samples were collected at GD20,and liver coefficients were calculated.Inductively coupled plasma mass spectrometry was used to detect the elemental contents in serum and liver;liquid chromatography tandem triple quadrupole mass spectrometry was used for pseudo-targeted lipidomics;and a fully automated biochemical analyzer was used to detect serum biochemical parameters.The results showed that high-dose As exposure induced disorders of lipid metabolism in rats,and Se intervention altered the process of lipid metabolism in serum.
作者
邹晓杰
何俐
蔡丽静
王妍
郑能兴
丁蕊
吴衍
ZOU Xiaojie;HE Li;CAI Lijing;WANG Yan;ZHENG Nengxing;DING Rui;WU Yan(School of Public Health,Fujian Medical University,Fuzhou 350122,China;School of Public Health,Xiamen University,Xiamen 361102,China;College of Environmental and Resource Sciences/College of Carbon Neutral Modern Industry,Fujian Normal University,Fuzhou 350117,China;Fujian Key Laboratory of Pollution Control&Resource Reuse,Fuzhou 350117,China)
出处
《福建师范大学学报（自然科学版）》
CAS
2023年第4期19-28,共10页
Journal of Fujian Normal University：Natural Science Edition
基金
国家自然科学基金青年基金资助项目(22006016)
福建省自然科学基金资助项目(2022J01710)。
关键词
砷
硒
脂代谢
脂质组学
arsenic
selenium
lipid metabolism
lipidomics
