摘要
目的:探讨幽门螺旋杆菌(Hp)阳性胃癌组织中微小核糖核酸-1290(miR-1290)、微小核糖核酸-134(miR-134)的表达及其与肿瘤增殖基因、侵袭基因和预后的关系。方法:选取2016年1月至2017年1月重庆大学附属三峡医院收治的126例胃癌患者为研究对象,根据Hp检测结果分为Hp阳性胃癌组(n=84)和Hp阴性胃癌组(n=42),另同期60例慢性胃炎患者为对照组。实时荧光定量聚合酶链反应检测各组组织中miR-1290、miR-134、增殖基因[磷脂酰肌醇3催化亚基A(PIK3CA)、C-myc癌基因(c-myc)]和侵袭基因[碱性螺旋环-螺旋转录因子(Twist)、N钙黏素(N-cad)]的表达。Pearson相关分析Hp阳性胃癌组织miR-1290、miR-134与肿瘤增殖PIK3CA、c-myc和侵袭基因Twist、N-cad表达的相关性,比较不同临床病理特征Hp阳性胃癌患者miR-1290、miR-134表达差异。Kaplan-Meier生存曲线评估miR-1290、miR-134表达与Hp阳性胃癌患者生存预后的关系。结果:Hp阳性胃癌组患者癌组织miR-1290表达高于Hp阴性胃癌组癌组织及对照组胃粘膜组织,miR-134表达低于Hp阴性胃癌组癌组织及对照组胃粘膜组织,差异有统计学意义(P<0.05)。Hp阳性胃癌组织中miR-1290表达与增殖基因PIK3CA、c-myc和侵袭基因Twist、N-cad mRNA表达呈正相关性(r=0.620~0.725,均P<0.05),miR-134与增殖基因PIK3CA、c-myc和侵袭基因Twist、N-cad mRNA表达呈显著负相关性(r=-0.670~-0.784,均P<0.05)。不同肿瘤TNM分期、浸润深度及淋巴结转移Hp阳性胃癌患者癌组织中miR-1290、miR-134表达比较,差异具有统计学意义(P均<0.05)。Hp阳性胃癌组患者中,miR-1290高表达组和低表达组5年总体生存率分别为48.78%(20/41),75.61%(31/41)。miR-1290高表达组患者累积生存率明显低于miR-1290低表达组(Log rankχ^(2)=6.366,P=0.012)。miR-134低表达组和高表达组患者5年总体生存率分别为42.86%(18/42),82.50%(33/40)。miR-134低表达组累积生存率显著低于miR-134高表达组(Log rankχ^(2)=10.640,P=0.001)。结论:Hp阳性胃癌组织miR-1290表达增加,miR-134表达降低,两者可能通过促进肿瘤增殖和侵袭基因的表达,导致肿瘤恶性进展及不良预后。
Objective:To investigate the expression of microRNA-1290(miR-1290)and miR-134 in helicobacter pylori(Hp)positive gastric cancer tissues and their relationship with tumor proliferation genes,invasion genes and prognosis.Methods:126 patients with gastric cancer who were admitted to Three Gorges Hospital Affiliated to Chongqing University from January 2016 to January 2017 were selected as the study subjects.According to the Hp detection results,they were divided into Hp-positive gastric cancer group(n=84)and Hp-negative gastric cancer group(n=42),and 60 patients with chronic gastritis in the same period were taken as the control group.The expression of miR-1290,miR-134,proliferation genes[phosphatidylinositol 3 catalytic subunit A(PIK3CA),C-myc oncogene(c-myc)]and invasion genes[basic helix helix transcription factor(Twist),N cadherin(N-cad)]were detected by real-time fluorescence quantitative polymerase chain reaction.Pearson correlation analysis of the correlation between miR-1290,miR-134 and the expression of PIK3CA,c-myc and invasive genes Twist and N-cad in Hp-positive gastric cancer tissues.The expression differences of miR-1290 and miR-134 in patients with Hp positive gastric cancer with different clinicopathological characteristics were compared.Kaplan-Meier survival curve was used to evaluate the relationship between the expression of miR-1290 and miR-134 and the survival and prognosis of Hp-positive patients with gastric cancer.Results:The expression of miR-1290 in cancer tissues of the HP-positive gastric cancer group was higher than that of the HP-negative gastric cancer group and control group,while the expression of miR-134 was lower than that of the HP-negative gastric cancer group and control group,with statistical significance(P<0.05).The expression of miR-1290 of the HP-positive gastric cancer tissues were positively correlated with the expressions of proliferating genes PIK3CA and c-myc,invasion genes Twist and N-cad mRNA(r-0.620~0.725,all P<0.05),and miR-134 was negatively correlated with the expressions of proliferating genes PIK3CA and c-myc,and invasion genes Twist and N-cad mRNA(r-0.670~-0.784,all P<0.05).There were statistically significant differences in the expression of miR-1290 and miR-134 in cancer tissues of patients with dfferent TNM stages,depth of invasion and lymph node metastasis of HP-positive gastric cancer(all P<0.05).The 5-year overall survival rates of patients with HP-positive gastric cancer were 48.78%(20/41)and 75.61%(31/41)of the high-expression group and low-expression group of miR-1290,respectively.The cumulative survival of patients with miR-1290 high expression group was significantly lower than that of patients with miR-1290 low expression group(Log rank χ^(2)=6.366,P=0.012).The 5-year overall survival rates were 42.86%(18/42)and 82.50%(33/40)in the low-expression group and high-expression group of miR-134,respectively.The cumulative survival of miR-134 low expression group was significantly lower than that of miR-134 high expression group(Log rank χ^(2)=10.640,P-0.001).Conclusion:The increased expression of miR-1290 and decreased expression of miR-134 in HP-positive gastric cancer tissues may lead to malignant progression and poor prognosis by promoting the expression of tumor proliferation and invasion genes.
作者
李亮
潘文琴
孙雪峰
张美
王镇澜
LI Liang;PAN Wen-qin;SUN Xue-feng;ZHANG Mei;WANG Zhen-lan(Department of Gastroenterology,Three Gorges Hospital Affiliated to Chongqing University,Chongqing,404000,China;Health Management Center,Three Gorges Hospital Affiliated to Chongqing University,Chongqing,404000,China;Department of Anorectal,Three Gorges Hospital Affiliated to Chongqing University,Chongqing,404000,China)
出处
《现代生物医学进展》
CAS
2023年第15期2888-2893,共6页
Progress in Modern Biomedicine
基金
重庆市科卫联合医学科研项目(2019MSXM211)。
