摘要
采用“52×3双水相试验法”优化双水相萃取条件,分别探究鲍鱼内脏、扇贝内脏和生蚝内脏双水相下相萃取物中的β-葡萄糖苷酶的酶活性和萃取效果。结果表明,采用该试验方法优化所得的临界体系为12%PEG4000/15%NaH_(2)PO_(4),以该体系萃取获得的下相萃取物中β-葡萄糖苷酶在酶活力、酶比活、纯化倍数和酶活回收率大小为生蚝内脏>鲍鱼内脏>扇贝内脏。
The enzyme activity and extraction effect ofβ-glucosidase in the aqueous two-phase subphase extracts of abalone viscera,scallop viscera and oyster viscera were investigated by using“52×3 aqueous two-phase extraction method”to optimize the extraction conditions.The results showed that the critical system optimized by this method was 12%PEG4000/15%NaH_(2)PO_(4).Theβ-glucosidase of subphase extracts extracted by this system showed the trend of oyster viscera>abalone viscera>scallop viscera in terms of enzyme activity,specific activity,purification ratio,and recovery.
作者
曾颖
曾淑欣
何玉书
林韫琦
ZENG Ying;ZENG Shuxin;HE Yushu;LIN Yunqi(Xiamen Medical College,Xiamen 361021,Fujian,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第16期100-105,共6页
Food Research and Development
基金
厦门医学院“大学生创新创业训练计划”资助项目(X202212631020)。
关键词
鲍鱼内脏
52×3双水相试验法
临界萃取
Β-葡萄糖苷酶
萃取效果
abalone viscera
52×3 aqueous two-phase extraction method
critical extraction
β-glucosidase
extraction effect
