摘要
通过EM菌剂、A菌剂以及A+EM菌剂启动污泥生物干化系统,探究不同菌剂对污泥生物干化系统启动过程中有机质尤其是蛋白质降解过程的影响.实验结果表明,不同菌剂接种的生物干化系统中蛋白质的降解程度呈现明显差异,EM组、A组、A+EM组蛋白质含量分别降低25.4%、20.1%、34.2%,菌剂A+EM组蛋白质降解最为迅速.蛋白质高效降解为微生物提供丰富的氮源,使得A+EM组总糖、脂肪降解量达至最高的63.1%和30.2%.此外,在A+EM组有机物高效降解的同时,污泥堆体的腐殖化进程呈现了减缓趋势.微生物分析结果表明,与其他两组相比,菌剂A+EM组中与升温相关的嗜热菌(≥50℃)丰度在高温期显著增加;参与蛋白质降解的氨化细菌与氨化古菌丰度均高于其它两组.结合代谢途径分析结果,EM和A两种菌剂的复配加强了生物干化系统中柠檬酸、糖酵解、磷酸戊糖循环,促进了蛋白质向氨氮的转化以及微生物对氨基酸、葡萄糖等物质的产生与利用,这是腐殖化进程减缓的重要原因之一.
Microbial agent EM,microbial agent A,and microbial agent A+EM were used to start the experiment of sludge biological drying systems.The influence of different microbial agents on the degradation of organic matters,especially proteins,during the startup of sludge biological drying systems was exploded.The degree of protein degradation was significantly different in the biodrying systems inoculated with different microbial agents.The protein content of EM group,A group and A+EM group decreased by 25.4%,20.1%,and 34.2%,and the protein degradation of A+EM group was the most rapid.The efficient protein degradation provided abundant nitrogen sources for microorganisms,which promoted the degradation of polysaccharides and fats in A+EM group with the highest of 63.1% and 30.2%.In addition,the organic matters in A+EM group degraded efficiently,and the humification process of sludge pile showed a retarding trend.Compared with the other two groups,the abundance of thermophilic bacteria(≥50℃)was related to the increase of temperature in the A+EM group during the thermophilic period.Especially,the abundance of ammonia-oxidizing bacteria and archaea involved in protein degradation were higher than those in the other two groups.Meanwhile,the results of metabolic pathway analysis indicated the combination of the two microbial agents strengthened tricarboxylic acid,glycolysis,and pentose phosphate cycles.The conversion of protein to ammonia nitrogen and the production and utilization of amino acids such as glucose was aslo promoted by microorganisms,which was one of the important reasons for slowing down the humification process.
作者
张锋
李军华
李宁
苏明雪
ZHANG Feng;LI Jun-hua;LI Ning;SU Ming-xue(School of Chemistry and Chemical Engineering,Hefei University of Technology,Hefei 230009,China;Hefei Cement Research&Design Institute Corporation Ltd,Hefei 230022,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2023年第8期4065-4079,共15页
China Environmental Science
基金
安徽省自然科学基金资助项目(2008085ME1611)
国家重点研发计划项目(2020YFC1908700)。
关键词
污泥
生物干化
代谢调控
群落结构
蛋白质
sludge
biodrying
metabolic control
community structure
protein
