摘要
以红颜草莓匍匐茎的茎尖为外植体,研究外植体的灭菌方法,并采用生长点诱导途径,探讨在基本MS培养基中加入植物生长调节剂(6-BA,NAA,IBA)、活性炭、白糖等对其生长点的诱导,丛芽的增殖、生根等关键环节的影响,以期建立草莓组培快繁技术体系。结果表明:紫外灯照射1 h,酒精消毒30 s,0.1%的HgCl2灭菌2 min灭菌效果最好;草莓生长点的诱导培养基为MS+6-BA 0.6 mg/L+白糖30 g/L+卡拉胶7.8 g/L,萌芽率达83.3%;增殖培养基为MS+6-BA 2 mg/L+NAA 0.1 mg/L+白糖30 g/L+卡拉胶7.8 g/L,增殖系可达6以上;生根培养基为3/4 MS+IBA 0.3m g/L+白糖20 g/L+卡拉胶7.8 g/L,植株生长良好,根系粗壮,生根较多。
We studied the sterilization method of strawberries,using stolon tips as explants,and used the growth point induc-tion route to explore the effects of adding plant growth regulators(6-BA,NAA,IBA),activated charcoal,and white sugar to the basic MS medium on the vital aspects of growth point induction,bush proliferation and rooting,to establish a strawberry group culture fast breeding technology system.The results showed that UV light irradiation for 1 h,disinfection with alcohol for 30 s and sterilization with 0.1%HgCl2 for 2 min was the most effective.The induction medium for strawberry growing point was MS+6-BA 0.6mg·L–1+white sugar 30 g·L–1+carrageenan 7.8 g·L–1,and the germination rate reached 83.3%;the proliferation medium was MS+6-BA 2 mg/L+NAA 0.1 mg·L–1+white sugar 30 g·L–1+carrageenan 7.8 g·L–1,and the prolif-eration coefficient could reach more than 6;the rooting medium was 3/4 MS+IBA 0.3m g/L+white sugar 20 g·L–1+carra-geenan 7.8 g·L–1,the plants grew well,with stronger roots and more roots.
作者
饶宝蓉
RAO Baorong(Nanping Institute of Agricultural sciences,Nanping,Fujian 354200,China)
出处
《热带农业科学》
2023年第6期38-44,共7页
Chinese Journal of Tropical Agriculture
基金
福建省财政厅(No.闽财农指[2022]23号)。
关键词
草莓
组织培养
正交设计
生根
strawberry
tissue culture
orthogonal design
rooting
