摘要
由茄科劳尔氏菌Ralstonia solanacearum引起的烟草青枯病是烟叶生产上的重要病害之一,严重影响烟叶的产量和品质。为筛选高效防治烟草青枯病的药剂,本研究采用平板菌落计数法测定了中生菌素、土霉素、噻霉酮、春雷霉素和氯溴异氰尿酸对茄科劳尔氏菌的抑制活性,并通过Biolog GENⅢ微平板法分析了上述5种药剂胁迫下茄科劳尔氏菌的碳代谢情况和对化学物质的敏感性差异。结果表明:5种药剂均能抑制茄科劳尔氏菌的生长,抑制活性从强到弱依次为中生菌素>土霉素>噻霉酮>春雷霉素>氯溴异氰尿酸,对应的EC_(50)值分别为0.24、0.74、2.84、7.95和273.99 mg/L。Biolog GENⅢ碳代谢测定结果显示:茄科劳尔氏菌能利用Biolog GENⅢ微平板中糖类及氨基酸类等71种碳源,但在药剂胁迫下,该菌对碳源的代谢受到了不同程度的抑制,其中抑制程度最显著的是氨基酸、己糖酸、羧酸、酯和脂肪酸类碳水化合物;随着5种药剂质量浓度增加,在6 mg/L中生菌素、8 mg/L土霉素、30 mg/L春雷霉素和5738 mg/L氯溴异氰尿酸胁迫下,茄科劳尔氏菌对Biolog GENⅢ微平板中65、18、60和7种碳源的代谢强度分别降低,同时对6、53、10和60种碳源的代谢强度增强;在8 mg/L噻霉酮胁迫下,茄科劳尔氏菌对Biolog GENⅢ微平板中71种碳源的代谢强度均降低。此外,在不同浓度的5种药剂胁迫和无药剂处理时,茄科劳尔氏菌对Biolog GENⅢ微平板中23种化学物质的敏感性不同,其对低浓度NaCl敏感性较低,而对低pH值更敏感。本研究结果可为烟草青枯病化学防控药剂的选择及其高效利用提供参考。
Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the important diseases in tobacco production,which seriously affects the yield and quality of tobacco leaves.In order to screen out efficient chemical agents for controlling tobacco bacterial wilt,the inhibitory activity of zhongshengmycin,oxytetracycline,benziothiazolinone,kasugamycin and chlorobromoisocyanuric acid on R.solanacearum was determined by plate colony counting method.The Biolog GEN III Microplate was used to investigate the carbon metabolism and chemical susceptibility of R.solanacearum under the stress of the above five chemicals.The results showed that R.solanacearum was restrained by all the five chemicals,and their antibacterial activity from strong to weak was zhongshengmycin>oxytetracycline>benziothiazolinone>kasugamycin>chlorobromoisocyanuric acid with EC_(50) values of 0.24,0.74,2.84,7.95 and 273.99 mg/L,respectively.The Biolog GEN III carbon metabolism showed that all the 71 carbon sources such as monosaccharides and amino acids in the Biolog GEN III Microplate could be metabolized by R.solanacearum.However,under chemical stress,the metabolism of carbon sources by R.solanacearum was inhibited to varying degrees,with amino acids,hexoic acids,carboxylic acids,esters and fatty acid carbohydrates being the most significantly inhibited.As the concentration of the tested chemicals increased,R.solanacearum reduced the metabolic intensity of 65,18,60,and 7 carbon sources in the Biolog GEN III Microplate under 6 mg/L of zhongshengmycin,8 mg/L of oxytetracycline,30 mg/L of kasugamycin and 5738 mg/L of chlorbromoisocyanuric acid stress,and increased 6,53,10,and 60 carbon sources,respectively.Under the stress of 8 mg/L benziothiazolinone,the metabolic intensity of 71 carbon sources of R.solanacearum decreased.In addition,the susceptibility of R.solanacearum to 23 chemicals in the Biolog GEN III Microplate differed at different concentrations of the five chemicals stress and without chemical treatment,with greater sensitivity to low pH and less sensitivity to low concentrations of NaCl.This study can provide a reference for the selection and efficient utilization of chemical control agents to tobacco bacterial wilt.
作者
罗飞
穆青
余知和
孙美丽
郭涛
李文红
汪汉成
蔡刘体
LUO Fei;MU Qing;YU Zhihe;SUN Meili;GUO Tao;LI Wenhong;WANG Hancheng;CAI Liuti(College of Life Sciences,Yangtze University,Jingzhou 434025,Hubei Province,China;Guizhou Academy of Tobacco Sciences,Guiyang 550081,China;Qianxinan Branch of Guizhou Tobacco Company,Xingyi 562400,Guizhou Province,China;Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei Province,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2023年第4期870-877,共8页
Chinese Journal of Pesticide Science
基金
中国烟草总公司贵州省公司科技项目(201914,2020XM03)
贵州省烟草公司黔西南州公司科技项目[州烟(2021)4号2021-06]
中国烟草总公司科技项目(110202101048(LS-08))
贵州省“百层次”创新型人才项目(GCC[2022]028-1).
关键词
抗生素
茄科劳尔氏菌
烟草青枯病
细菌代谢
碳代谢
抑菌活性
Biolog
GENⅢ
antibiotics
Ralstonia solanacearum
tobacco bacterial wilt
bacterial metabolism
carbon metabolism
inhibitory activity
Biolog GENⅢ
