摘要
天冬氨酸酶(aspartase,Asp)是一种裂合酶,能够催化L-天冬氨酸与富马酸间的可逆反应,在微生物氮代谢中发挥着重要作用。天冬氨酸酶也是一种重要的生物催化剂,可用于L-天冬氨酸及其衍生物、β-丙氨酸等氨基酸的合成。综述了细菌中天冬氨酸酶的结构、理化性质及催化机理,讨论了天冬氨酸酶在制备L-天冬氨酸及其衍生物、β-丙氨酸的进展。天冬氨酸酶的结构及催化机理的解析,为扩大其底物范围,生产种类丰富的L-天冬氨酸衍生物奠定了理论基础。
Aspartase,a type of lyase,plays an important role in microbial nitrogen metabolism by catalyzing the reversible reaction between L-aspartic acid and fumaric acid.Aspartase is also an important biocatalyst,which can be used for L-aspartic acid and its derivatives,synthesis of amino acids such as β-alanine.The structure,physicochemical properties,and catalytic mechanism of aspartase in bacteria are comprehensively reviewed in this paper.In addition,the progress in the preparation of L-aspartic acid and its derivatives,as well as β-alanine,using aspartase is discussed.The analysis of the structure and catalytic mechanism of aspartase lays a theoretical foundation for expanding its substrate range and producing a variety of L-aspartic acid derivatives.
作者
李冉
贾振华
宋聪
张翔
黄媛媛
LI Ran;JIA Zhenhua;SONG Cong;ZHANG Xiang;HUANG Yuanyuan(Institute of Biology,Hebei Academy of Sciences,Shijiazhuang Hebei 050051,China)
出处
《河北省科学院学报》
CAS
2023年第4期16-21,33,共7页
Journal of The Hebei Academy of Sciences
基金
河北省科学院科技计划项目(2020031506)。