摘要
目的:建立测定淫羊藿中淫羊藿苷、朝藿定A、朝藿定B和朝藿定C等4个黄酮醇苷含量的超高效液相色谱一标多测分析方法。方法:采用超高效液相色谱,以淫羊藿苷为内参物,建立朝藿定A、朝藿定B和朝藿定C与淫羊藿苷之间的校正因子,并考察校正因子在不同条件下的稳定性,同时比较一标多测法(QAMS)和外标法(ESM)测定结果的差异,验证QAMS的准确性。结果:4个化合物平均回收率分别为100.95%、99.33%、100.30%、98.03%,RSD分别为1.45%、1.14%、0.77%、1.45%。该方法在不同实验条件下耐用性良好;朝藿定A、朝藿定B和朝藿定C 3个化合物相对于淫羊藿苷的校正因子分别为1.21、1.17、1.31,两种分析方法所得结果基本无差异。结论:所建立的QAMS简便、快捷、准确度高,对控制淫羊藿的质量具有参考价值。
Objective:To establish a ultra performance liquid chromatography(UPLC)method for determination of four flavonol glycosides in Epimedium brevicornu by quantitative analysis of multi-components by single-marker(QAMS).Methods:The correction factors of epimedin A,epimedin B and epimedin C were generated by UPLC method with icariin as an internal standard,and the stability of the correction factors under different conditions was investigated.Moreover,the accuracy of QAMS was verified by comparing the determination results of QAMS and external standard method(ESM).Results:The average recovery of four compounds was 100.95%,99.33%,100.30%,98.03%with the RSD of 1.45%,1.14%,0.77%,1.45%,respectively.The established method had good applicability under different experimental conditions,and the correction factors of epimedin A,epimedin B and epimedin C with icariin as a reference were 1.21,1.17 and 1.31,respectively.Furthermore,no significant difference in the results obtained by QAMS and ESM was observed.Conclusion:The established QAMS method is simple,rapid and highly accurate,and provide a reference for the quality evaluation of E.brevicornu.
作者
韩志岭
高云佳
莫梦
张庆英
屠鹏飞
HAN Zhiling;GAO Yunjia;MO Meng;ZHANG Qingying;TU Pengfei(State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China;SinoPharm Pharmaceuticals Limited,Beijing 102206,China)
出处
《中国药品标准》
CAS
2023年第4期411-417,共7页
Drug Standards of China
基金
国家重点研发计划(2018YFC1707904,2018YFC1707900)。
关键词
淫羊藿
一标多测
超高效液相色谱
淫羊藿苷
黄酮醇苷
Epimedium brevicornu
quantitative analysis of multi-components by single-marker(QAMS)
ultra performance liquid chromatography(UPLC)
icariin
flavonol glycosides
