摘要
目的:建立一种高效液相色谱法检测保定市在售饼干、面包、派等食品中安赛蜜的方法,监测食品安全风险,保证消费品安全。方法:采用C18反相色谱柱,二极管阵列检测器,以0.02 mol·L^(-1)乙酸铵溶液∶甲醇=95∶5(V∶V)为流动相,检测波长225 nm,流速1.0 mL·min-1,温度28.1℃,检测环境湿度58%,以水为提取溶剂超声波多次提取安赛蜜,加入沉淀剂去除提取液中杂质,进样量5μL,上机测定安赛蜜含量。结果:共检测了27份样品,最低检出限为0.001μg·mL^(-1),相对标准偏差为0.45%,样品回收率在95%以上,超标数0,合格率100%。结论:本方法操作简单、快捷、准确率较高,适合饼干、面包、派等烘焙食品中安赛蜜含量的监测。
Objective:To establish a high performance liquid chromatography method for the detection of acesulfame potassium in biscuits,bread,pie and other foods sold in Baoding city,so as to monitor food safety risks and ensure the safety of consumer products.Method:The analysis was performed on a C18 reversed-phase column with diode array detector.The mobile phase was 0.02 mol·L^(-1) ammonium acetate solution∶methanol=95∶5(V∶V).The detection wavelength was 225 nm,the flow rate was 1.0 mL·min^(-1),the temperature was 28.1℃,and the humidity was 58%.The acesulfame potassium was extracted by ultrasonic with water as the extraction solvent.The precipitant was added to remove the impurities in the extract.The injection volume was 5μL,and the content of acesulfame potassium was determined by machine.Result:A total of 27 samples were tested,with a minimum detection limit of 0.001μg·mL^(-1),a relative standard deviation of 0.45%,and sample recovery rate above 95%,exceed the standard 0,qualified rate 100%.Conclusion:This method is simple and fast to operate,and has high accuracy.It is suitable for monitoring acesulfame potassium content in biscuits,bread,pie and other baked goods.
作者
赵迪
ZHAO Di(Henan Vocational University of Science Technology,Zhoukou 466000,China)
出处
《食品安全导刊》
2023年第22期100-102,共3页
China Food Safety Magazine
关键词
安赛蜜
高效液相色谱
食品添加剂
acesulfame potassium
high performance liquid chromatography
food additive
