摘要
目的探究银杏素(GK)对压力超负荷心肌肥厚小鼠的心脏保护作用。方法C57BL/6J小鼠(8周龄)24只,通过主动脉弓缩窄(TAC)建立小鼠压力超负荷心肌肥厚,随机分为手术组(n=8)、手术+GK组(n=8)和假手术组(n=8)。手术组不做处理,手术+GK组于术后每日给予GK治疗,假手术组经历同样手术操作,但不进行70线打结。通过小鼠心脏超声评估小鼠心脏收缩功能,通过心脏超声、心脏质量与胫骨长度比值、WGA染色和Myh7的转录水平等评估心肌肥厚程度,通过肺脏质量与体质量比值、血清脑钠肽(BNP)水平和Nppb的转录水平等评估术后心力衰竭程度,最后通过Masson染色及纤维化相关分子的转录水平评估心脏的纤维化程度。结果GK显著改善了TAC手术引起的小鼠心脏射血分数下降(P<0.05)和短轴缩短率的下降(P<0.01);降低了TAC术后小鼠心脏室间隔厚度(P<0.05)和左心室后壁厚度(P<0.05);减小了心脏质量与胫骨长度比值(P<0.05);减小了TAC术后心肌细胞横截面积(P<0.01)及肥厚相关基因Myh7的转录水平(P<0.01)。GK显著减小了TAC术后小鼠肺脏质量与体质量的比值(P<0.05),降低了小鼠血清中BNP的含量(P<0.01),同时降低了左心室心肌组织中Nppb的转录水平(P<0.05)。最后,GK显著减轻了TAC术后小鼠心脏纤维化程度(P<0.01),降低了小鼠心脏组织中纤维化相关分子Tgfb1(P<0.01)、Col1a1(P<0.05)和Col3a1(P<0.05)的转录水平。结论GK具有改善TAC术后小鼠心脏功能、减轻心肌肥厚和心力衰竭以及减轻心肌纤维化的作用。
Objective To investigate the cardioprotective effect of ginkgetin(GK)on pressure overload-induced myocardial hypertrophy in mice.Methods Pressure overload-induced myocardial hypertrophy was established by transverse aortic constriction(TAC)in mice.Twenty-four C57BL/6J mice(8 weeks old)were randomly divided into TAC group(n=8),TAC+GK group(n=8)and Sham group(n=8).The TAC group was not treated,the TAC+GK group was given GK every day after operation,and the Sham group experienced the same operation without 70 line tied.Echocardiography was used to evaluate the cardiac systolic function of mice.Echocardiography,the ratio of heart weight to tibial length,WGA staining,and the transcription level of Myh7 were applied to evaluate the degree of myocardial hypertrophy.The ratio of lung weight to body weight,serum brain natriuretic peptide(BNP)level,and the transcription level of Nppb were used to evaluate the degree of heart failure after operation.Finally,cardiac fibrosis was evaluated by Masson staining and the transcription level of fibrosis-related molecules.Results GK significantly improved the decrease of cardiac ejection fraction(P<0.05)and fractional shortening(P<0.01),decreased the thickness of interventricular septum(P<0.05)and left ventricular posterior wall(P<0.05),reduced the ratio of heart weight to tibial length(P<0.05),and decreased the cross-sectional area of cardiomyocytes(P<0.01)and the transcription level of hypertrophy-related gene Myh7(P<0.01)in mice after TAC.GK significantly reduced the ratio of lung weight to body weight(P<0.05),decreased the content of BNP in serum(P<0.01),and also reduced the transcription level of Nppb in left ventricular myocardial tissue in mice after TAC(P<0.05).Finally,GK significantly reduced the degree of cardiac fibrosis in mice after TAC(P<0.01),and reduced the transcription levels of fibrosis-related molecules Tgfb1(P<0.01),Col1a1(P<0.05),and Col3a1(P<0.05)in cardiac tissue of mice.Conclusion GK can improve cardiac function,reduce the degree of myocardial hypertrophy and heart failure,and alleviate myocardial fibrosis in mice after TAC.
作者
石广永
张冰
赵琳
陈亚武
金振晓
徐学增
SHI Guangyong;ZHANG Bing;ZHAO Lin;CHEN Yawu;JIN Zhenxiao;XU Xuezeng(Department of Cardiovascular Surgery,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
出处
《空军军医大学学报》
CAS
2023年第8期700-705,共6页
Journal of Air Force Medical University
基金
国家自然科学基金青年科学基金(82000227)。
关键词
银杏素
压力超负荷
心肌肥厚
心脏功能
ginkgetin
pressure overload
myocardial hypertrophy
cardiac function
