摘要
苎麻是一种重要的纤维作物,纤维被用作纺织工业的原料。扩展蛋白具有诱导依赖pH的细胞壁伸长和压力松弛的特性。为了对苎麻基因组中expansin家族成员数量和类型进行鉴定,并研究在纤维细度不同的苎麻茎皮中扩展蛋白基因的表达情况,研究首先在苎麻中苎1号基因组数据库挖掘到24个扩展蛋白基因家族成员,通过生物信息学方法对其分子量、等电点、信号肽、亚细胞定位、motif及基因结构进行分析和预测。系统进化树结果显示:扩展蛋白包括α亚族成员17个、β亚族4个、α类亚族1个和β类亚族2个,共分为3类;expansin家族同一进化支蛋白结构域比较保守且有一个特有的motif。然后,利用苎麻茎皮扩展蛋白基因家族成员转录组表达分析和qRT-PCR验证,发现两个基因在两个纤维细度差异显著的品种及其5个不同生长期表达量差异显著。该结果有助于了解苎麻expansin家族的进化,为影响苎麻纤维发育和纤维细度相关基因及其功能的研究奠定基础。
Ramie is an important fiber crop,and fiber is used as the raw material of textile industry.Expansin have the character of inducing PH-dependent cell wall elongation and stress relaxation.In order to explore the number and type of the expansin family members in ramie genome and to study the expression pattern of expansin genes in the stem bark of ramie varieties with different fiber finesse,24 members of the expansin gene family were firstly detected in ramie from the Zhongzhu No.1 genomic database.Analysis and prediction of protein molecular weight,isoelectric point,signal peptide and subcellular localization were conducted on the family members using bioinformatics in this study.By constructing the phylogenetic tree,17 Expansinαsubfamily,4 Expansinβsubfamily,1 Expansinα-like subfamily and 2 Expansinβ-like subfamilies members were divided into 3 categories.In same evolutionary branch of expansin family,protein domain is relative conservative and the protein sequences of each gene subfamily has a specific motif.Expression pattern of expansin gene family members in stem bark of ramie was analyzed using transcriptome data and significant expression difference of two genes was confirmed at five growth stages of the two varieties through qRT-PCR.The results are helpful for understanding the evolution of the ramie expansin gene family,as well as future studies of genes and their functions that affect fiber development and fiber finesse.
作者
石亚亮
钟意成
黄坤勇
牛娟
孙志民
陈建华
栾明宝
SHI Yaliang;ZHONG Yicheng;HUANG Kunyong;NIU Juan;SUN Zhimin;CHEN Jianhua;LUAN Mingbao(National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572024,Hainan,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410221,Hunan,China)
出处
《中国麻业科学》
2023年第4期145-151,共7页
Plant Fiber Sciences in China
基金
国家自然科学基金(31671744)
2060299-2-23年科技创新工程-基础研究-南繁育种中心-麻类作物专用品种繁育及种质创新项目(ZDXM2306)。
