摘要
为研究壮药三七姜抗氧化能力及其化学成分,本文通过DPPH、O_(2)^(-)·、·OH自由基清除实验,超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)技术,对三七姜抗氧化能力及化学成分进行分析。结果表明:三七姜不同极性部位均具有一定的抗氧化能力,乙酸乙酯部位抗氧化能力最强;三七姜中含有莪术醇、莪术二酮、樟脑、α-香附酮、壬二酸、柠檬酸、原儿茶醛、异夏佛塔苷、尿苷、L-酪氨酸、烟酰胺、L-色氨酸、甘露醇、丁烯基苯酞等14个化合物,均为首次从三七姜中鉴定出来。研究表明三七姜具有一定的抗氧化活性,原儿茶醛、烟酰胺等成分可能是其直接发挥药效作用的物质,该研究为三七姜的开发利用提供参考依据。
In order to study the antioxidant activity and composition of Zhuang medicine Stahlianthus involucratus,the antioxidant capacity and chemical composition of Stahlianthus involucratus were analyzed through DPPH、O_(2)^(-)·and·OH free radical scavenging experiments and UPLC-Q-Orbitrap HRMS.The results showed that different extracts of Stahlianthus involucratus had certain antioxidant effect,and the ethyl acetate extracts had the strongest antioxidant ability.In total,14 compounds were identified from Stahlianthus involucratus for the first time,including curcumol,curdione,α-cyperone,azelaic acid,citric acid,protocatechuic aldehyde,isoschaftoside,uridine,L-tyrosine,nicotinamide,L-tryptophan,mannitol and 3-butylidenephthalide.There is a certain antioxidant capacity in the Stahlianthus involucratus,protocatechuic aldehyde,nicotinamide and other components may be the substances that directly play the key role.This study provides a reference for the development and utilization of Stahlianthus involucratus.
作者
梁洁
刘星晨
孙正伊
陈晓思
杨川川
曹玉嫔
李耀华
陈明伟
黄团心
LIANG Jie;LIU Xingchen;SUN Zhengyi;CHEN Xiaosi;YANG Chuanchuan;CAO Yupin;LI Yaohua;CHEN Mingwei;HUANG Tuanxin(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China;South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation(Guangxi University of Chinese Medicine),Nanning Guangxi 530200,China;Key Laboratory of TCM Extraction and Purification and Quality Analysis(Guangxi University of Chinese Medicine),Nanning Guangxi 530200,China;Education Department of Guangxi Zhuang Autonomous Region,Guangxi Zhuang Yao Medicine Center of Engineering and Technology(Guangxi University of Chinese Medicine),Nanning Guangxi 530200,China;Department of Pharmacy,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning Guangxi 530011,China;Guangxi Institute for Food and Drug Control,Nanning Guangxi 530021,China)
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2023年第4期165-177,共13页
Journal of Guangxi Normal University:Natural Science Edition
基金
广西自然科学基金(2020GXNSFAA238026)
广西壮瑶药重点实验室项目(GXZYZZ2020-05、桂科基字〔2014〕32号)
广西一流学科建设项目(桂教科研〔2018〕12号)
广西科技基地和人才专项(桂科AD20238058)
壮瑶药协同创新中心项目(桂教科研〔2013〕20号)
广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号)
广西中医药重点学科壮药学(GZXK-Z-20-64)
第八批广西壮族自治区特聘专家项目(桂人才通字〔2019〕13号)
广西高等学校千名中青年骨干教师培育计划(桂教人〔2019〕5号)。
