摘要
随着生物技术和胚胎工程的不断发展,在牦牛体外受精、胚胎生物技术等方面不断探索,为牦牛资源的育种、保护、基因改良提供有利条件。牦牛卵母细胞是这些技术最重要、最基础的资源和载体,其体外成熟和体外受精效果是体外胚胎生产的基础环节和关键。该文综述近年牦牛卵母细胞来源、体外培养、精子体外获能、体外受精及早期胚胎培养环节的研究进展,并对影响不同环节的主要因素进行概括,对牦牛卵母细胞体外成熟与体外受精在胚胎体外生产中的应用提出展望。
With the development of biotechnology and embryo engineering,in vitro fertilization and embryo biotechnology of yak have been explored,providing favorable conditions for breeding,protection and gene improvement of yak resources.Yak oocyte is the most important and basic resource and carrier of these technologies,and its effect in vitro maturation and in vitro fertilization are the basic link and key of in vitro embryo production.In this paper,the research progress of yak oocyte source,in vitro culture,in vitro capacitation of sperm,in vitro fertilization and early embryo culture in recent years was reviewed,and the main factors affecting different processes were summarized,and the application of in vitro maturation and in vitro fertilization of yak oocyte in vitro embryo production was put forward.
作者
张犇
郭宪
ZHANG Ben;GUO Xian(Lanzhou Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences,Key Laboratory of Qinghai Tibet Plateau Livestock and Poultry Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Key Laboratory of Gansu Yak Breeding Project,Lanzhou Gansu 730050,China)
出处
《现代畜牧科技》
2023年第8期97-101,共5页
Modern Animal Husbandry Science & Technology
基金
国家自然科学基金(32272852)
财政部和农业农村部—现代农业(肉牛牦牛)产业技术体系建设专项(CARS-37)
中国农业科学院科技创新工程牦牛资源与育种(25-LZIHPS-01)。
关键词
牦牛
卵母细胞
体外成熟
体外受精
yak
oocyte
in vitro maturation
in vitro fertilization
