摘要
比色生物传感技术由于具有灵敏度高、方法简单并且容易操作等优点,已广泛应用于生物环境中污染物检测、生物体内重要标志物的检测以及癌症筛查等多个领域。基于纳米酶的比色生物传感器主要是借助纳米酶自身所具有的催化能力,模拟类过氧化物酶活性,将显色剂氧化生成有色溶液,从而实现可视化检测,并通过对有色溶液吸光度的检测得到相关物质的含量。与无纳米酶的比色生物传感器相比,基于纳米酶的比色生物传感器具有选择性更高、检测更快以及灵敏度更高等优点。纳米酶在具有天然酶活性的同时还具有成本低、稳定性好的、易于合成等优点,其相关研究越来越广泛。目前,基于纳米酶的比色生物传感器已成为辅助相关医学检测的重要方法,同时也广泛应用于便携和实时性相关检测当中,为医学检测提供了重要的支持和保障。为了提高比色生物传感器的灵敏度以及应用范围,研究人员也在致力于增加可检测物质的种类以及纳米酶种类的多样化等。本文主要介绍基于纳米酶的比色生物传感器的检测原理、几类典型的纳米酶,以及基于纳米酶的比色生物传感器在生物医学检测领域中的应用情况和研究进展。
Because of its advantages of high sensitivity,simple method and easy operation,colorimetric biosensing technology has been widely used in many fields such as the detection of pollutants in the biological environment,the detection of important markers in the organism and cancer screening.The colorimetric biosensor based on nanozymes mainly uses the catalytic ability of nanozymes to simulate peroxidase-like activities,oxidize the chromogenic agent to form a colored solution,so as to realize visual detection,and obtain the content of related substances through the detection of its absorbance.Compared with colorimetric biosensors without nanozymes,colorimetric biosensors based on nanozymes have the advantages of higher selectivity,faster detection and higher sensitivity.Nanozymes,on the other hand,are more and more widely studied for their natural enzyme activity while also having the advantages of low cost,good stability,and easy synthesis.At present,colorimetric biosensors based on nanozymes have become an important method to assist related medical detection,and are also widely used in portable and real-time related detection,providing important support and guarantee for medical detection.In order to improve the sensitivity and application range of colorimetric biosensors,researchers are also working on increasing the variety of substances to be detected and diversifying the types of nanozymes.This paper mainly introduces the detection principle of nanozyme-based colorimetric biosensors,several typical used nanozymes,and the application and research progress of nanozyme-based colorimetric biosensors in the field of biomedical detection.
作者
袁琳
陆冬筱
李金华
YUAN Lin;LU Dong-Xiao;LI Jin-Hua(Nanophotonics and Biophotonics Key Laboratory of Jilin Province,College of Physics,Changchun University of Science and Technology,Changchun 130022,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2023年第7期1638-1650,共13页
Progress In Biochemistry and Biophysics
基金
吉林省科技发展计划(20210101155JC)
国家自然科学基金(62174015,62171115)
教育部“111”创新引智项目(D17017)
吉林省教育厅项目(JJKH20200730KJ,JJKH20210798KJ)资助
