摘要
目的 利用超高效液相色谱-串联质谱仪建立一种快速、准确检测禽肉和禽蛋中硝基咪唑类药物(替硝唑、奥硝唑、络硝哒唑、甲硝唑和二甲硝唑)残留的新方法。方法 样品经三氯乙酸提取,提取液浓缩后酸性溶液溶解,采用MCX混合阳离子固相萃取柱净化,采用超高效液相色谱-串联质谱测定,基质匹配内标法定量。结果 本方法硝基咪唑类药物检出限为:1.0μg/kg,定量限为:3.0μg/kg。目标检测物在5.0~50μg/L浓度范围内线性关系良好,且3个添加浓度加标回收率大于70%,相对偏差小于20%。结论 该方法高效简便、准确可靠,可应用于禽肉及禽蛋中硝基咪唑类药物残留的检测。
Objective To establish a new method for rapid and accurate determination of nitroimidazoles(including tinidazole,ornidazole,ronidazole,metronidazole,and dimethylmetronidazole)residues in poultry meat and eggs by using ultra-high performance liquid chromatography tandem mass spectrometry.Methods The samples were extracted with trichloroacetic acid,and then the extraction solution was concentrated and dissolved in acid solution.Before use of ultra-high performance liquid chromatography tandem mass spectrometry for the analysis,the extraction solution was purified by a mixed-mode cation exchange(MCX)solid-phase extraction column,and quantification of the detection data was performed by internal standard method.Results In this method,the detection and quantitation limits of nitroimidazoles were 1.0μg/kg and 3.0μg/kg respectively.A good linear relationship was obtained with the concentrations ranged from 5.0μg/L to 50μg/L,the spiked recoveries were above 70%and the relative deviation was less than 20%with three different spiked concentrations.Conclusion The method is efficient,simple,accurate and reliable,which can be used for the determination of nitroimidazole residues in poultry meat and eggs.
作者
汪琼
严付华
白祖海
胡骢
WANG Qiong;YAN Fuhua;BAI Zuhai;HU Cong(Changsha Municipal Center for Disease Control and Prevention,Changsha,Hunan 410004,China)
出处
《实用预防医学》
CAS
2023年第7期833-835,共3页
Practical Preventive Medicine
关键词
硝基咪唑
禽肉禽蛋
超高效液相色谱-串联质谱
甲硝唑
nitroimidazole
poultry meat and egg
high-performance liquid chromatography tandem mass spectrometry
metronidazole
