摘要
以不同茶树(Camellia sinensis)基因组为参考,通过序列同源性分析,在‘龙井43’中鉴定并克隆到3个CsIDM基因。CsIDM1属于组蛋白乙酰转移酶家族,具有典型的HAT保守结构域,定位于细胞核中。CsIDM2、CsIDM3均属于热激蛋白家族,具有典型的ACD保守结构域,均定位于细胞核、细胞膜及细胞质中。CsIDM启动子区域含有多种响应环境信号的顺式作用元件,主要为光响应、植物激素响应、胁迫响应和植物生长发育相关。CsIDM在花蕾、腋芽、茎中表达量较高,在盛花组织中较低。在干旱、高温及生物胁迫下,CsIDM均出现不同程度的差异表达。茶树越冬芽休眠形成与解除过程中,CsIDM1的表达丰度在休眠形成、深休眠和休眠解除3个阶段存在高—低—高的表达模式。通过双分子荧光互补试验证实,CsIDM2和CsIDM3之间可形成异源二聚体;CsIDM2与Cs MBD5、CsIDM3与CsMBD16存在相互作用。综上所述,CsIDM在茶树的胁迫应答和芽休眠解除中发挥作用,可能通过形成蛋白复合体参与甲基化调控。
In this study,using different tea plant genomes as a reference,three CsIDM genes were identified and cloned from Camellia sinensis‘Longjing 43’.CsIDM1,belongs to the histone acetyltransferase family,has a typical HAT conserved domain,and is located in the nucleus.CsIDM2 and CsIDM3,which belong to the heat shock protein family,have typical ACD conserved domains,which are located in the nucleus,cell membrane and cytoplasm.The promoter region of CsIDMs contains a variety of cis-acting elements that respond to environmental signals,including light response,plant hormone response,stress response and plant growth-related elements.Expression analysis showed that CsIDMs expressed higher in flower buds,axillary buds and stems,but lower in fully open flowers,which suggests a certain tissue specificity.CsIDMs were differentially expressed under drought,high temperature and biotic stresses.During the formation and release of dormancy in overwintering buds,the expression of CsIDM1 showed a high-low-high expression pattern in the stages of paradormancy,endodormancy and ecodormancy.It was confirmed that CsIDM2 interacts with CsIDM3 to form a heterodimer by bimolecular fluorescence complementation.CsIDM2 and CsIDM3 interact with CsMBD5 and CsMBD16,respectively.In summary,CsIDMs play an important role in the stress response and bud dormancy removal of tea plants,and may participate in the regulation of methylation through the formation of protein complexes.
作者
李宇腾
陈瑶
任恒泽
李聪聪
王浩乾
曹红利
岳川
郝心愿
王新超
LI Yuteng;CHEN Yao;REN Hengze;LI Congcong;WANG Haoqian;CAO Hongli;YUE Chuan;HAO Xinyuan;WANG Xinchao(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;National Center for Tea Improvement,Tea Research Institute Chinese Academy of Agricultural Sciences,Hangzhou 310008,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第8期1679-1696,共18页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31972461,32172632)
浙江省农业新品种(茶树)重大科技专项(2021C02067-2)
国家现代农业产业技术体系建设专项资金项目(CARS-19)
中国农业科学院基本科研业务费专项(Y2021PT07)。
关键词
茶树
去甲基化
IDM复合物
亚细胞定位
表达分析
BiFC互作
Camellia sinensis
demethylation
IDM complex
subcellular localization
expression analysis
BiFC assay
