摘要
目的:观察羊肠线、聚乙交酯丙交酯(PGLA)线埋入健康大鼠“足三里”穴区对局部皮肤肥大细胞(MC)激活及P物质(SP)、组胺(HA)表达的影响,初步探讨穴位埋线时效性刺激效应的机制,为深入研究穴位埋线源头启动机制奠定基础。方法:将160只SPF级雄性SD大鼠随机分为空白组(10只)、空刺组(50只)、羊肠线组(50只)和PGLA线组(50只),各干预组再随机分为干预后8 h、3 d、7 d、14 d、21 d5个时间点亚组,每亚组10只。各干预组于左侧“足三里”分别实施埋线针空刺、羊肠线埋入、PGLA线埋入1次。空白组于干预前1 d、其余组分别于干预后对应时间点取左侧“足三里”穴区皮肤及皮下结缔组织,采用甲苯胺蓝染色法检测MC计数及脱颗粒情况,采用免疫组化法检测SP、HA阳性细胞表达。结果:各干预组亚组MC计数与空白组比较,差异无统计学意义(P>0.05);羊肠线组、PGLA线组各亚组间MC计数比较差异均无统计学意义(P>0.05);PGLA线埋入后8 h组MC计数多于羊肠线埋入后8 h组(P<0.05),PGLA线埋入后21 d组MC计数少于羊肠线埋入后21 d组(P<0.05)。与空白组比较,空刺后8 h、3 d组,羊肠线埋入后8 h、3 d、7 d组,PGLA线埋入后8 h、3 d、7 d、14 d组MC脱颗粒率均升高(P<0.01,P<0.05,P<0.001);羊肠线组、PGLA线组各亚组间MC脱颗粒率比较差异均无统计学意义(P>0.05);两埋线组同时间点MC脱颗粒率组间比较,差异均无统计学意义(P>0.05)。与空白组比较,空刺后8 h组,羊肠线埋入后8 h、3 d、7 d、14 d组,PGLA线埋入后3 d、7 d、14 d组SP阳性细胞表达均增加(P<0.001,P<0.05)。羊肠线埋入后7 d组SP阳性细胞表达多于羊肠线埋入后8 h组(P<0.05),羊肠线埋入后14 d组SP阳性细胞表达少于羊肠线埋入后7 d组(P<0.001);PGLA线埋入后7 d组SP阳性细胞表达多于PGLA线埋入后3 d组(P<0.05),PGLA线埋入后14 d组SP阳性细胞表达少于PGLA线埋入后7 d组(P<0.01)。两埋线组同时间点SP阳性细胞表达组间比较,差异无统计学意义(P>0.05)。与空白组比较,空刺后8 h、3 d组,羊肠线埋入后8 h、3 d、7 d、14 d组,PGLA线埋入后8 h、3 d、7 d、14 d、21 d组HA阳性细胞表达均增加(P<0.001,P<0.01,P<0.05)。羊肠线埋入后14 d组HA阳性细胞表达少于羊肠线埋入后7 d组(P<0.05);PGLA线埋入后3 d组HA阳性细胞表达多于PGLA线埋入后8 h(P<0.05),PGLA线埋入后14 d组HA阳性细胞表达少于PGLA线埋入后7 d组(P<0.05)。PGLA线埋入后3 d组HA阳性细胞表达多于羊肠线埋入后3 d组(P<0.05)。结论:羊肠线、PGLA线埋入健康大鼠“足三里”穴区可引起局部皮肤MC、SP及HA变化,可能是埋线后穴位局部时效性刺激效应的机制之一,且不同线体之间有一定差异;以MC介导、SP及HA共同参与的穴区局部适度炎性反应可能是穴位埋线效应的源头启动环节之一。
Objective To observe the effects of catgut embedding and polyglycolic acid/poly-lactic acid(PGLA)embedding at"Zusanli"(ST 36)on the activation of local skin mast cells(MC),and expression of substance P(SP)and histamine (HA), and to explore the mechanism of the temporal stimulation effect of acupoint catgut embedding andprovide a foundation for further research on the initiation mechanism of acupoint catgut embedding. Methods Onehundred and sixty male SPF-grade SD rats were randomly divided into a blank group (10 rats), a sham-embedding group(50 rats), a catgut group (50 rats), and a PCLA group (50 rats). Each intervention group was further randomly divided intofive subgroups according to the time points after intervention: 8 hours, 3 days, 7 days, 14 days, and 21 days, with 10 ratsin each subgroup. One-time sham-embedding, catgut embedding and PGLA embedding was given at left "Zusanli" (ST 36)in each intervention group, respectively. The skin and subcutaneous connective tissue of the left "Zusanli" (ST 36) werecollected at the corresponding time points after intervention, except for the blank group (only one day before intervention).Toluidine blue staining was used to detect MC count and degranulation, and immunohistochemical staining was used todetect the expression of SP and HA positive cells. Results There was no significant difference in MC count between thesubgroups of each intervention group and the blank group (P>0.05). There was no significant difference in MC countbetween the subgroups of the catgut group and the PGLA group (P> 0.05). The MC count in the 8-hour subgroup of PGLAgroup was higher than that in the 8-hour subgroup of catgut group (P<0.05), while the MC count in the 21-day subgroupof PGLA group was lower than that in the 21-day subgroup of catgut group (P< 0.05). Compared with the blank group, thedegranulation rates of MC were increased in the 8-hour and 3-day subgroups of sham-embedding group, 8-hour, 3-day,and 7-day subgroups of catgut group, and 8-hour, 3-day, 7-day, and 14-day subgroups of PGLA group (P< 0.01, P<0.05,P<0.001). There was no significant difference in the degranulation rate of MC between the subgroups of the catgut groupand the PCLA group (P> 0.05), and no significant difference in the degranulation rate of MC between the two embeddinggroups at the same time point (P>0.05). Compared with the blank group, the expression of SP positive cells wasincreased in the 8-hour subgroup of sham-embedding group, 8-hour, 3-day, 7-day, and 14-day subgroups of catgut group,and 3-day, 7-day, and 14-day subgroups of PGLA group (P< 0.001, P< 0.05). The expression of SP positive cells in the7-day subgroup of catgut group was higher than that in the 8-hour subgroup of catgut group (P<0.05), while the expression of SPpositive cells in the 14-day subgroup of catgut group was lower than that in the 7-day subgroup of catgut group (P<0.001). Theexpression of SP positive cells in the 7-day subgroup of PGLA group was higher than that in the 3-day subgroup of PGLAgroup (P<0.05), while the expression of SP positive cells in the 14-day subgroup of PCLA group was lower than that inthe 7-day subgroup of PGLA group (P< 0.01). There was no significant difference in the expression of SP positive cellsbetween the subgroups of the two embedding groups at the same time point (P> 0.05). Compared with the blank group, theexpression of HA positive cells was increased in the 8-hour, 3-day subgroups of sham-embedding group, 8-hour, 3-day, 7-day,and 14-day subgroups of catgut group, and 8-hour, 3-day, 7-day, 14-day, and 21-day subgroups of PGLA group (P<0.001,P<0.01, P< 0.05). The expression of HA positive cells in the 14-day subgroup of catgut group was lower than that inthe 7-day subgroup of catgut group (P< 0.05), while the expression of HA positive cells in the 3-day subgroup ofPCLA group was higher than that in the 8-hour subgroup of PGLA group (P< 0.05), and the expression of HA positivecells in the 14-day subgroup of PGLA group was lower than that in the 7-day subgroup of PGLA group (P< 0.05). Theexpression of HA positive cells in the 3-day subgroup of PGLA group was higher than that in the 3-day subgroup of catgutgroup (P<0.05). Conclusion Catgut and PGLA embedding at "Zusanli" (ST 36) in healthy rats could induce changes inlocal skin MC, SP, and HA, which may be one of the mechanisms of the temporal stimulation effect after acupointembedding. There are certain differences between different suture materials. A moderate inflammatory response in theacupoint area, mediated by MC and involving SP and HA, may be one of the initiating factors for the effect of acupointcatgut embedding.
作者
王钰嘉
李丽红
侯珣瑞
聂红昉
梁欣
张倩
程玲
叶婷婷
WANG Yu-jia;LI Li-hong;HOU Xun-rui;NIE Hong-fang;LIANG Xin;ZHANG Qian;CHENG Ling;YE Ting-ting(Department of Acupuncture and Moxibustion,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;Clinical Medicine College of Guizhou Medical University)
出处
《中国针灸》
CAS
CSCD
北大核心
2023年第8期944-950,共7页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金资助项目:81960894
贵州医科大学国家自然科学基金培育项目:20NSP021。
关键词
穴位埋线
穴
足三里
肥大细胞
P物质
组胺
时效性刺激
炎性反应
acupoint catgut embedding
Point ST 36(Zusanli)
mast cell(MC)
Substance P(SP)
histamine(HA)
temporal stimulation
inflammatory response
