摘要
以花椒叶为材料,花椒叶多糖得率作为考察指标,优化双水相体系提取花椒叶多糖的工艺参数,并分析其对亚硝酸盐的清除活性。结果表明:最佳工艺参数为多糖提取液体积6.1 mL、K_(2)HPO_(4)用量2.2 g、PEG6000用量2.7 g,在此条件下花椒叶多糖的得率为10.092 mg/g,与预测值相差较小;亚硝酸盐清除活性试验表明,花椒叶多糖对亚硝酸盐有一定的清除作用,且存在量效关系。
Using pepper leaves as the material and the yield of pepper leaves polysaccharides as the evaluation index,the process parameters for extracting pepper leaves polysaccharides by a two-phase aqueous system were optimized,and its nitrite scavenging activity was analyzed.The results showed that the optimal process parameters were 6.1 mL of polysaccharides extraction solution volume,2.2 g of K_(2)HPO_(4) dosage,and 2.7 g of PEG6000 dosage.Under these conditions,the yield of polysaccharides from pepper leaves was 10.092 mg/g,which had a small difference from the predicted value.The nitrite scavenging activity test showed that the polysaccharides from pepper leaves had a certain scavenging effect on nitrite,and there was a dose-effect relationship.
作者
段鸿斌
吴晓辉
孙伟
叶润
蔡静
DUAN Hong-bin;WU Xiao-hui;SUN Wei;YE Run;CAI Jing(School of Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;Xinyang Comprehensive Administrative Enforcement Detachment of Market Supervision,Xinyang 464000,Henan,China)
出处
《粮食与油脂》
北大核心
2023年第9期70-74,共5页
Cereals & Oils
基金
信阳农林学院“十四五”校级科技创新团队项目(XNKJTD-009)
河南省科技攻关项目(222102110247)。
关键词
花椒叶
双水相体系
多糖
响应面设计
亚硝酸盐
pepper leaf
aqueous two-phase system
polysaccharide
response surface design
nitrite
