摘要
该研究以3-氨基苯硼酸(3-APBA)为原料,通过一步水热法制备了荧光量子产率为51.43%的硼掺杂碳点(B-CDs),该B-CDs呈典型球状,平均粒径为5.8 nm。研究发现所制备的B-CDs的荧光发射峰与2,4,6-三硝基苯酚(TNP)的紫外-可见吸收峰明显重叠,且两者之间存在内滤效应(IFE)。基于IFE机理,以BCDs为荧光探针建立了TNP的快速分析方法。所建方法在0.5~100μmol/L范围内呈现良好的线性关系,检出限(3σ)为86 nmol/L。该分析方法成功应用于环境水样中TNP的测定,实际样品的加标回收率为95.1%~110%,相对标准偏差(RSD)为1.0%~3.9%。基于IFE机理的TNP分析方法具有简便、灵敏、选择性高的特点。
2,4,6-Trinitrophenol(TNP)is a commonly used nitroaromatic explosive.Excessive use will cause serious pollution to soil and groundwater.TNP not only causes a series of environmen⁃tal problems,but also has many negative effects on the human body.So far,many analytical meth⁃ods have been applied to the determination of nitroaromatic explosives,such as high performance liq⁃uid chromatography(HPLC),mass spectrometry(MS),and electrochemical methods.However,these methods have some drawbacks,such as high cost,complicated sample pretreatment,and ex⁃pensive instruments.It is of great significance to develop a simple,sensitive and selective method for the detection of TNP.In order to solve this problem,a rapid detection and analysis method of TNP was established based on the inner filter effect(IFE)using B-doped carbon dots(B-CDs)as fluo⁃rescent probes.In this study,B-CDs with fluorescence quantum yield of 51.43%were prepared by one-step hydrothermal method using 3-aminophenylboronic acid(3-APBA)as raw material.The mor⁃phology and particle size distribution of B-CDs were observed by transmission electron microscopy(TEM).The functional groups on the surface of B-CDs were investigated by FT-IR spectroscopy.The elemental composition and chemical state of B-CDs were studied by X-ray photoelectron spectros⁃copy(XPS).The optical properties of B-CDs were investigated by fluorescence spectroscopy and ul⁃traviolet-visible absorption spectroscopy.In addition,the effects of pH,salt concentration and tem⁃perature on the fluorescence intensity of B-CDs were studied.It was found that the fluorescence emis⁃sion peak of the prepared B-CDs overlapped with the ultraviolet-visible absorption peak of TNP.Ac⁃cording to the fluorescence lifetime results,it was judged that there was an internal filtering effect be⁃tween the two.The pH 6.0 and room temperature were selected as the optimal experimental condi⁃tions.Under the optimal experimental conditions,the fluorescence intensity of B-CDs decreased reg⁃ularly in the range of 0.5-100μmol/L TNP concentration.The TNP concentration showed a good lin⁃ear relationship with the quenching degree lg(I0/I),and the linear equation was lg(I0/I)=0.01c+0.02286(r^(2)=0.99978).The detection limit was calculated to be 86 nmol/L according to the 3σrule.The method was successfully applied to the determination of TNP in environmental water sam⁃ples.The spiked recoveries of the actual samples were 95.1%-110%,and the relative standard devi⁃ation(RSD)was in the range of 1.0%-3.9%.The TNP analysis method based on IFE mechanism is simple,sensitive and selective.
作者
李俊丽
何梦瑶
闫新雨
向国强
江秀明
何丽君
LI Jun-li;HE Meng-yao;YAN Xin-yu;XIANG Guo-qiang;JIANG Xiu-ming;HE Li-jun(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2023年第9期1127-1133,共7页
Journal of Instrumental Analysis
基金
国家粮食和物资储备局粮油食品工程技术研究中心开放课题(GA2018007)。
关键词
碳点
硼掺杂
内滤效应
2
4
6-三硝基苯酚
水
carbon dots
boron doping
inner filter effect
2,4,6-trinitrophenol
water
