摘要
为获得高效的辣椒根腐病生防菌,从健康辣椒根际土壤中分离出真菌菌株,通过与辣椒根腐病病原菌茄镰刀菌(Fusarium solani)进行对峙培养,筛选出对茄镰刀菌抑制率达到79.29%的生防菌株M20210527-2。结合形态学及ITS分子生物学分析,将菌株M20210527-2鉴定为哈茨木霉(Trichoderma harzianum)。菌株M20210527-2可通过重寄生、快速争夺生长空间以及分泌抗真菌代谢产物来高效抑制茄镰刀菌的生长。菌株M20210527-2处理对辣椒根腐病相对防效高达94.25%,可显著降低根腐病发病率和严重程度。培养条件研究表明,菌株M20210527-2最适生长温度为25℃,最佳pH为6~7,最适生长碳源为蔗糖、葡萄糖和淀粉,最适生长氮源为蛋白胨、酵母粉和牛肉膏。
In order to obtain high efficient bio-control fungus for pepper root rot,and isolate fungi strain from rhizosphere soil of healthy pepper plants,this paper conducted confrontation culture with Fusarium solani;and screened the antagonistic strain M20210527-2 with 79.29%inhibition rate on Fusarium solanum.Combined with morphology and ITS molecular biology analysis,strain M20210527-2 was identified as Trichoderma harzianum.Strain M20210527-2 could quickly compete for growth space and secrete antifungal metabolites for effective inhibition on Fusarium solani growth by hyperparasitism.Strain M20210527-2 treatment could relatively increase the control efficiency on pepper root rot as high as 94.25%and also significantly reduce its incidence and severity.Studies on culture conditions indicated that the optimum growth temperature for strain M20210527-2 was 25℃;optimums pH was 6-7;optimum growth carbon sources were sucrose,glucose,and starch;optimum growth nitrogen sources were peptone,yeast powder and beef extract.
作者
李树江
张韵霞
刘羽
周闯闯
张芝琴
冯道
杨友联
吴迪
张晓勇
LI Shujiang;ZHANG Yunxia;LIU Yu;ZHOU Chuangchuang;ZHANG Zhiqin;FENG Dao;YANG Youlian;WU Di;ZHANG Xiaoyong(School of Biological Sciences and Technology,Liupanshui Normal College,Liupanshui 553004,Guizhou,China;Liupanshui City Academy of Agricultural Sciences,Liupanshui 553000,Guizhou,China)
出处
《中国蔬菜》
北大核心
2023年第9期69-76,共8页
China Vegetables
基金
贵州省教育厅2023年度普通本科高校科学研究项目(青年项目)(黔教技[2022]337号)
六盘水师范学院2022年一流本科专业建设点项目(2022-03-040)
2018年度六盘水师范学院项目(LPSSYjxtd201802),2020年贵州省大学生创新创业训练计划项目(S202010977029)
六盘水市省地科技合作项目(52020-2016-HK-06)。
关键词
辣椒根腐病
茄镰刀菌
生防真菌
哈茨木霉
鉴定
培养特性
pepper root rot
Fusarium solani
bio-control fungi
Trichoderma harzianum
identification
cultural characteristics
