摘要
本研究旨在了解宁夏地区肉牛病毒性腹泻病原感染现状及流行特点,为牛腹泻病的防控工作提供科学依据。本研究采用RT-PCR方法对宁夏地区293份肉牛拭子样本进行牛病毒性腹泻病毒(BVDV)、牛冠状病毒(BCoV)、牛轮状病毒(BRV)、牛诺瓦病毒(BNoV)、牛星状病毒(BAstV)检测,并对其进行遗传进化分析。研究表明,该地区肉牛普遍存在腹泻病毒的感染及混合感染;散养模式下肉牛腹泻病毒的感染及混合感染情况较规模化养殖严重;病毒性腹泻在不同地区、不同病毒差异明显;遗传进化分析发现宁夏地区BVDV流行株为1e亚型,BRV流行株为G1亚型,BNoV流行株为GⅢ.2亚型,BCoV流行株与法国株遗传进化关系较近。结果显示,宁夏地区肉牛普遍存在腹泻相关病毒的感染,不同养殖模式、不同地区、不同病毒之间存在差异,且混合感染严重。
This study aimed to determine the current status and epidemiological characteristics of beef cattle viral diarrheal pathogen infection in Ningxia,and to provide scientific basis for the prevention and control of bovine diarrheal disease.In this study,293 beef cattle swabs in Ningxia were detected and genetically analyzed by RT-PCR for bovine viral diarrhea virus(BVDV),bovine coronavirus(BCoV),bovine rotavirus(BRV),bovine norovirus(BNoV),bovine astrovirus(BAstV),and analyzed their genetic evolution.The Studies showed that diarrhoeal virus infection and mixed infection were prevalent in beef cattle;The infection and mixed infection of beef cattle diarrhea virus in free-range breeding mode were more serious than those in large-scale breeding.Viral diarrhea varied significantly between different regions and viruses;Genetic evolution analysis showed that the BVDV epidemic strain in Ningxia was subtype 1e,the BRV epidemic strain was G1 subtype,the BNoV epidemic strain was GIII.2,and the BCoV epidemic strain was closely related to the genetic evolution of the French strain.The results showed that diarrheal virus infection was widespread in beef cattle in Ningxia,which was different between different breeding modes,different regions and different viruses,and the mixed infection was serious.
作者
姜玲玲
牛小霞
刘强
张刚
王璞
李勇
JIANG Lingling;NIU Xiaoxia;LIU Qiang;ZHANG Gang;WANG Pu;LI Yong(Ministry of Education Key Laboratory of Western Characteristic Biological Resources Protection and Utilization,Ningxia University,Yinchuan 750021,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2023年第9期3863-3871,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
宁夏回族自治区重点研发计划项目资助课题(2021BEF02028)
国家自然科学基金区域联合基金项目(32130104)。
关键词
肉牛
腹泻相关病毒
RT-PCR检测
遗传进化分析
beef cattle
diarrhea-related viruses
RT-PCR detection
genetic evolution analysis
