摘要
目的 探究靶向高通量测序(targeted next-generation sequencing,tNGS)技术在疑似肺炎患者支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)样本中的病原学诊断价值。方法 选取2022年7月~2023年3月武汉亚心总医院收治的102例疑似肺炎患者的102份BALF样本,对其同时进行了tNGS检测和传统微生物学检测,并收集患者的一般临床资料。对纳入研究的102例患者的tNGS检测结果、微生物培养结果以及其他实验室检查结果进行回顾性分析。结果 tNGS法的总体阳性检出率(93.14%)高于传统方法(58.82%),差异有统计学意义(χ^(2)=32.903,P<0.001);t NGS法在细菌、病毒以及混合感染方面的检出率(65.69%,55.89%,69.61%)高于传统方法(21.57%,6.86%,11.76%),差异有统计学意义(χ^(2)=32.903,56.920,70.708,均P<0.001)。结论 tNGS技术在疑似肺炎患者BALF样本中获得了较高的病原体检出率,可以有效应用于呼吸道感染性疾病的病原学诊断。
Objective To explore the value of targeted next-generation sequencing(tNGS)in etiological diagnosis of bronchoalveolar lavagefluid(BALF)samples from patients with suspected pneumonia.Methods 102 samples of BALF samples from 102 suspected pneumonia patients admitted to Wuhan Asia General Hospital from July 2022 to March 2023 were selected.tNGS and traditional microbiology tests were performed on them at the same time,and general clinical data of the patients were collected.tNGS test results,microbial culture results and other laboratory results of 102 patients were retrospectively analyzed.Results The overall positive detection rate of tNGS method(93.14%)was higher than that of traditional method(58.82%),and the difference was statistically significant(χ^(2)=32.903,P<0.001).The detection rates of bacterial,viral and mixed infections by tNGS method(65.69%,55.89%,69.61%)were higher than those by traditional methods(21.57%,6.86%,11.76%),and the differences were statistically significant(χ^(2)=32.903,56.920,70.708,all P<0.001).Conclusion tNGS technique has a high pathogen detection rate in BALF samples of suspected pneumonia patients,and can be effectively used in the etiological diagnosis of respiratory infectious diseases.
作者
颜新生
张丹
王栋
张李涛
张真路
连姝文
YAN Xinsheng;ZHANG Dan;WANG Dong;ZHANG Litao;ZHANG Zhenlu;LIAN Shuwen(Department of Clinical Laboratory,Wuhan Asia General Hospital,Wuhan 430056,China;Department of Pharmacy,Wuhan Asia General Hospital,Wuhan 430056,China)
出处
《现代检验医学杂志》
CAS
2023年第5期12-16,共5页
Journal of Modern Laboratory Medicine
基金
武汉市卫生健康委员会青年项目(WX21Q29):利伐沙班血药浓度与高龄非瓣膜性房颤患者不良预后的关系研究。
关键词
靶向高通量测序
支气管肺泡灌洗液
病原体检测
targeted next-generation sequencing
bronchoalveolar lavagefluid
pathogen detection
