摘要
目的:探究miR-146a过表达在基于RhoA/ROCK通路对类风湿关节炎大鼠关节损伤的修复作用。方法:选取40只SD健康雄性大鼠。10只纳入正常组,其余30只建立类风湿关节炎大鼠模型,并分为模型组、沉默miR-146a组、过表达miR-146a组(每组10只)。正常组、模型组使用生理盐水干预,沉默miR-146a组建立沉默miR-146a模型,过表达miR-146a组建立过表达miR-146a模型。分别在研究3周、6周、9周、12周对各组大鼠进行关节炎指数(AI)评分。蛋白质印迹法检测RhoA、ROCK1、ROCK2及腺苷酸活化蛋白激酶(AMPK)、克隆叉头蛋白O3a(FoxO3a)蛋白表达情况。使用酶联免疫法检测白细胞介素-8(IL-8)、白细胞介素-17(IL-17)、超敏C反应蛋白(hs-CRP)水平。结果:沉默miR-146a组大鼠AI评分高于模型组;过表达miR-146a组大鼠AI评分低于模型组、沉默miR-146a组,但高于正常组(P<0.05)。模型组、沉默miR-146a组大鼠RhoA、ROCK1及ROCK2表达水平均低于正常组,且沉默miR-146a组高于模型组(P<0.05);过表达miR-146a组大鼠RhoA、ROCK1及ROCK2表达水平高于正常组,但低于模型组、沉默miR-146a组(P<0.05)。沉默miR-146a组大鼠IL-8、IL-17、hs-CRP水平均高于模型组,过表达miR-146a组大鼠IL-8、IL-17、hs-CRP水平均低于模型组、沉默miR-146a组,高于正常组(P<0.05)。模型组、沉默miR-146a组大鼠AMPK、磷酸化AMPK(p-AMPK)、FoxO3a、磷酸化FoxO3a(p-FoxO3a)蛋白表达水平低于正常组,沉默miR-146a组高于模型组,过表达miR-146a组大鼠高于正常组,低于模型组、沉默miR-146a组(P<0.05)。结论:基于RhoA/ROCK通路的miR-146a过表达对类风湿关节炎大鼠进行干预,可以显著改善大鼠疾病症状,修复关节损伤程度,降低炎症反应,有效抑制类风湿关节炎的发生发展,具有临床价值。
Objective:To explore the role of overexpression of miR-146a in the repair of joint injury caused by rheumatoid arthritis in rats based on RhoA/ROCK pathway.Methods:Forty healthy SD male rats were selected.Ten rats were selected as normal group and rheumatoid arthritis model was established in the other 30 rats which were divided into model group,miR-146a silencing group and miR-146a overexpression group(n=10).The normal group and model group were intervened with normal saline.The miR-146a model was established in miR-146a silencing group and miR-146a overexpression group.Arthritis index(AI)was used to evaluate the rats at 3,6,9,and 12 weeks of the study.The expression of RhoA,Rock1,Rock2,adenylate activated protein kinase(AMPK)and FOXO3a were detected by Western blot.The levels of IL-8,IL-17 and hs-CRP were detected by ELISA.Results:The AI scores of miR-146a silencing group were higher than those of model group(P<0.05).The AI scores of miR-146a overexpression group were lower than those of model group and miR-146a silencing group,but higher than those of normal group(P<0.05).The expression of RhoA,Rock1 and Rock2 in model group and miR-146a silencing group were lower than those in normal group,and the expression of RhoA,Rock1 and Rock2 in miR-146a silencing group was higher than those in model group(P<0.05).The levels of IL-8,IL-17 and hs-CRP in miR-146a group were higher than those in the model group.The levels of IL-8,IL-17 and hs CRP in miR-146a overexpression group were lower than those in the model group and miR-146a group,but higher than those in the normal group(P<0.05).The protein expressions of AMPK,p-AMPK,FOXO3a and p-foxo3a in model group and miR-146a silencing group were lower than those in normal group,and the protein expressions in miR-146a silencing group was higher than those in model group(P<0.05).The protein expressions of AMPK,p-AMPK,FOXO3a and p-foxo3a in miR-146a overexpression group were higher than those in normal group,but lower than those in model group and miR-146a silencing group(P<0.05).Conclusions:Based on RhoA/ROCK pathway,miR-146a overexpression can significantly improve the symptoms of rheumatoid arthritis rats,the repair of joint injury,reduce the inflammatory response,and effectively inhibit the occurrence and development of rheumatoid arthritis,which has clinical value.
作者
李刚
胡立新
徐昕
熊敏
LI Gang;HU Lixin;XU Xin;XIONG Min(Department of Sports Medicine,Dongfeng General Hospital of Traditional Chinese Medicine,Shiyan 442008,Hubei;Department of Spinal Surgery,Dongfeng General Hospital of Traditional Chinese Medicine,Shiyan 442008,Hubei,China)
出处
《中华骨与关节外科杂志》
CSCD
2023年第7期628-633,共6页
Chinese Journal of Bone and Joint Surgery
基金
十堰市科学技术研究与开发项目(18Y94)。
关键词
类风湿关节炎
关节损伤
腺苷酸活化蛋白激酶
炎症反应
Rheumatoid Arthritis
Joint Injury
Adenylate Activated Protein Kinase
Inflammatory Response
