摘要
目的对某新型冠状病毒定点医院临时新型冠状病毒核酸实验室进行核酸污染监测及生物安全风险评估。方法于2022年3月14日至5月16日每周一对某新型冠状病毒定点医院临时新型冠状病毒核酸实验室实验过程中以及消毒后各高风险实验活动区域及实验室工作人员防护物品表面进行新型冠状病毒核酸采样,采用实时荧光定量PCR技术对样本进行检测。结果各高风险实验活动区域及实验室工作人员防护物品表面共40处采样点采集760份样本。样本处理区有8个采样点检出阳性,阳性率为27%(27/100),其中以生物安全柜、转运箱外表面和编号样本架阳性检出率最高,分别为5/10、4/10和6/10。核酸检测区有7个采样点检出阳性,全部出现在PCR综合实验间,阳性率为7.1%(10/140),其中以样本传递窗和核酸检测区门把手阳性检出率最高,均为4/20。来自样本处理区的阳性样本靶基因Ct值显著高于核酸检测区。实验人员防护物品表面的检出阳性率为20%(16/80),部分实验人员在实验过程中外层手套阳性检出率最高可达9/10。消毒后新型冠状病毒核酸实验室各区域及实验人员防护物品表面的核酸残留能够被有效清除。结论实验过程中样本处理区物体表面新型冠状病毒核酸阳性检出率及污染强度高于核酸检测区,且实验人员在工作中面临较高生物安全风险。科学开展消毒流程有助于降低实验室环境及实验人员防护物品表面的核酸残留风险,从而保证检验质量和实验室人员安全。
Objective To monitor nucleic acid contamination and evaluate the biosecurity risk in a temporary 2019-nCoV nucleic acid testing laboratory situated within designated infectious disease care facilities.Methods Quantitative real-time PCR technology was used to detect nucleic acid contamination in samples collected from high-risk experimental activity areas and the surface of the laboratory staff′s personal protective equipment.Sampling was conducted every Monday from March 14 to May 16,2022,both during and after disinfection procedures.Results A total of 760 samples were collected from 40 sampling sites.A total of 27 out of 100 samples(27%)collected from 8 sampling sites in the sample processing area were positive.Among them,the contaminated area of biological safety cabinet,the outer surface of the sample transport box,and the sample rack were found to have the highest positive detection rates,with rates of 5/10,4/10,and 6/10,respectively.Ten out of 140 samples(7.1%)obtained from 7 sampling sites in the nucleic acid detection area showed positive results.The inner wall of sample transfer window and the door handle of the nucleic acid detection area had the highest positive detection rates,both at 4/20.The Ct values for the target genes from the positive samples in the sample processing area were significantly higher than those from the nucleic acid detection area.The detection rate for nucleic acid contamination on the surface of the personal protective equipment of the laboratory staff was 20%(16/80),and the positive detection rate of the outer gloves from operator during the experiment reached 9/10.After disinfection,the nucleic acid residues on the surfaces of the various areas of the 2019-nCoV nucleic acid laboratory and the surfaces of the personal protective equipment of the laboratory staff were observed to be effectively removed.Conclusions During experimental operation,the positive detection rate and nucleic acid contamination intensity of 2019-nCoV are higher in the sample processing area compared to those in the nucleic acid detection area.The laboratory staff are exposed to high biosecurity risk during the experiment.Implementing a scientific disinfection process can significantly reduce the risk of 2019-nCoV residues from the laboratory environment and the surface of the personal protective equipment of the laboratory staff,ensuring the accuracy of inspections and the safety of the laboratory staff.
作者
沈隽霏
孟志民
史庆丰
王冲
潘柏申
王蓓丽
郭玮
Shen Junfei;Meng Zhimin;Shi Qingfen;Wang Chong;Pan Baishen;Wang Beili;Guo Wei(Department of Laboratory Medicine,Zhongshan Hospital of Fudan University,Shanghai 200032,China;Administration department of nosocomial infection,Zhongshan Hospital of Fudan University,Shanghai 200032,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2023年第9期943-949,共7页
Chinese Journal of Laboratory Medicine
基金
国家自然基金(81972000,82172348,81902139)
复旦大学附属中山医院临床研究专项基金(2018ZSLC05,2020ZSLC54)
上海市临床重点专科建设项目(shslczdzk03302)
厦门市医疗卫生重点项目(YDZX20193502000002)。
