摘要
【背景】药用植物内生真菌产生的次生代谢产物具有多种抗菌活性,因而具有生防菌开发潜力。【目的】鉴定一株对葫芦科刺盘孢(Colletotrichum orbiculare)有抑菌活性的‘海螺’望春花内生真菌EF-WCH-51,并确定其主要抑菌活性成分。【方法】根据形态特征并结合分子序列鉴定EF-WCH-51菌株,利用生长速率法确定EF-WCH-51抑菌活性,并采用半制备型HPLC、HPLC-MS/MS对其活性成分进行分离鉴定。【结果】供试菌株EF-WCH-51为丝枝蜡蚧菌(Lecanicillium aphanocladii),其发酵液粗提物对葫芦科刺盘孢菌丝生长具有较强抑制作用,EC50为0.0862 mg/mL;抑菌活性组分A(0.0850 mg/mL)对葫芦科刺盘孢抑制率达到76.08%,通过查询MS、MS/MS数据库以及与相关文献对比,确定该化合物为卵孢菌素(oosporein)。【结论】本研究发现丝枝蜡蚧菌对葫芦科刺盘孢具有较好的抑菌活性,其主要抑菌活性成分为卵孢菌素,具有作为瓜类炭疽病生防菌株的潜力。
[Background]The endophytic fungi of medicinal plants have good biocontrol potential because the secondary metabolites they produced have a variety of antimicrobial activities.[Objective]To identify an endophytic fungus EF-WCH-51 with inhibitory activity against Colletotrichum orbiculare(a pathogen causing anthracnose of melons)from Yulania denudata‘Hailuo’and determine its main antifungal component.[Methods]Strain EF-WCH-51 was identified based on morphological characteristics and molecular sequences.The growth rate method was employed to determine the antifungal activity of EF-WCH-51,and semi-preparative HPLC and HPLC-MS/MS to identify the active component.[Results]Strain EF-WCH-51 was identified as Lecanicillium aphanocladii.The crude extract of its fermentation broth had a strong inhibitory effect on the mycelial growth of C.orbiculare,with EC50 of 0.0862 mg/mL.The inhibitory rate of the bioactive compound A(0.0850 mg/mL)against C.orbiculare was 76.08%.The compound was identified as oosporein by data comparison with MS,MS/MS databases and related literature.[Conclusion]L.aphanocladii,with oosporein as the main antifungal active component,had good inhibitory activity against C.orbiculare,demonstrating the potential as a biocontrol strain against C.orbiculare.
作者
张义军
王捷
裴宁宁
秦丽
王士娟
彭凡
ZHANG Yijun;WANG Jie;PEI Ningning;QIN Li;WANG Shijuan;PENG Fan(Anhui Provincial Key Laboratory of Microbial Control,School of Forestry&Landscape Architecture,Anhui Agricultural University,Hefei 230036,Anhui,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2023年第9期3997-4009,共13页
Microbiology China
基金
安徽省林业科技攻关团队项目(2019[15])
安徽省大学生创新训练计划(S202010364090)。
关键词
‘海螺’望春花
内生真菌
葫芦科刺盘孢
丝枝蜡蚧菌
卵孢菌素
Yulania denudata‘Hailuo’
endophytic fungi
Colletotrichum orbiculare
Lecanicilium aphanocladii
oosporein
