摘要
为研究不同生长方式及年限对防风品质的影响,通过色差仪量化防风样品粉末表观颜色,计算总色值(E^(*)ab),采用HPLC同时测定样品中升麻素苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷和3′-O-当归酰亥茅酚含量,利用多元统计方法对样品粉末色度值与5种成分含量进行聚类分析、主成分分析、偏最小二乘法判别分析及Pearson相关性分析。结果表明防风样品粉末总色值(E^(*)ab)野生防风<多年生防风<一年生防风;野生防风的升麻素、亥茅酚苷和3′-O-当归酰亥茅酚含量显著高于多年生防风及一年生防风;多元统计分析结果显示,多年生防风质量差异大,其品质虽不及野生防风,但接近野生防风,优于一年生防风;3′-O-当归酰亥茅酚含量、E^(*)ab、b^(*)、L^(*)和升麻素含量的变量投影重要性(VIP)均>1,升麻素含量的VIP亦接近于1;升麻素苷、升麻素、亥茅酚苷和3′-O-当归酰亥茅酚含量与E^(*)ab呈显著负相关,而5-O-甲基维斯阿米醇苷含量与E^(*)ab不存在线性相关。生长方式及年限对防风品质有影响,防风粉末色度值与5种成分能客观判定防风的品质,可考虑将3′-O-当归酰亥茅酚和升麻素作为防风生长过程控制的质量标志物。
TThis study aims to reveal the effects of different growth patterns and years on the quality of Saposhnikoviae Radix samples.The apparent colors of the powder samples were quantified by a colorimeter,and the total color values(E^(*)ab)were calculated.The content of prim-O-glucosylcimifugin,cimifugin,4'-O-β-D-glucosyl-5-O-methylvisamminol,sec-O-glucosylhamaudol,and 3'-O-angeloylhamaudol in the samples was simultaneously determined by high performance liquid chromatography(HPLC).Cluster analysis,principal component analysis,partial least squares discriminant analysis,and Pearson correlation analysis were performed to analyze the powder chromatic values and the content of 5 components.The results showed that the E^(*)ab values of the samples were in the order of wild group<multiple-year-old group<one-year-old group.The content of cimifugin,sec-Oglucosylhamaudol,and 3'-O-angeloylhamaudol in the wild group was significantly higher than that in the multiple-year-old and oneyear-old groups.The results of multivariate statistical analysis showed that the quality of multiple-year-old group varied greatly.The quality of the multiple-year-old samples was close to that of the wild group and better than that of the one-year-old group.The variable importance in the projection(VIP)values of b^(*),3'-O-angeloylhamaudol content,E^(*)ab,and L^(*)were all larger than 1,and that of cimifugin content was close to 1.The E"ab value was negatively correlated with the content of prim-O-glucosylcimifugin,cimifugin,sec-O-glucosylhamaudol,and 3'-O-angeloylhamaudol,while it had no linear correlation with the 4'-0-β-D-glucosyl-5-0-methylvisamminol content.The growth patterns and years had different effects on the quality of Saposhnikoviae Radix samples.The chromatic values of Saposhnikoviae Radix and the content of 5 components can be used to evaluate the quality of Saposhnikoviae Radix,and 3'-O-angeloylhamaudol and cinmifugin can be considered as markers for the quality control of Saposhnikovia divaricata during the growing process.
作者
杨琳琳
李千
王璇
程水清
文佳
王旭星
张海霞
徐新房
李向日
YANG Lin-lin;LI Qian;WANG Xuan;CHENG Shui-qing;WEN Jia;WANG Xu-xing;ZHANG Hai-xia;XU Xin-fang;LI Xiang-ri(Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Bejing 102488,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第15期4106-4114,共9页
China Journal of Chinese Materia Medica
基金
防风药材与饮片标准的研究项目(2021Z003)。
关键词
防风
生长方式
生长年限
色度值
升麻素
3′-O-当归酰亥茅酚
多元统计分析
Saposhnikoviae Radix
growth patterns
growth years
chromatic values
cimifugin
3′-O-angeloylhamaudol
multivariate statistical analysis
