摘要
以油莎豆膳食纤维作为发酵底物,并以大豆膳食纤维为对照,利用体外结肠发酵技术,并结合16S rRNA高通量测序,探究油莎豆膳食纤维对肠道菌群结构及短链脂肪酸(short-chain fatty acids,SCFAs)的影响。结果表明,油莎豆膳食纤维可显著促进SCFAs的产生,但产酸能力弱于大豆膳食纤维;高浓度的膳食纤维可显著改变肠道菌群的多样性和丰富度,且这2种膳食纤维对肠道菌群的影响具有显著差异;门水平结果显示,油莎豆膳食纤维可显著提高厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和放线菌门(Actinobacteria)的相对丰度,而抑制拟杆菌门(Bacteroidota)的生长,这与大豆膳食纤维对肠道菌群的作用略有不同;属水平结果显示,相比于大豆膳食纤维,油莎豆膳食纤维促进罕见小球菌属(Subdoligranulum)、双歧杆菌属(Bifidobacterium)、乳杆菌属(Lactobacillus)和粪杆菌属(Faecalibacterium)生长繁殖的能力更强,且显著抑制了普雷沃菌属(Prevotella)和拟杆菌属(Bacteroides)的生长。该研究为油莎豆膳食纤维成为一种新型益生元提供了一定的理论依据。
In this study,in vitro colonic fermentation and 16S rRNA high-throughput sequencing were used to explore the effects of the Cyperus esculentus dietary fiber on the production of short-chain fat acids(SCFAs)by the human gut microbiota and its structure.Soybean dietary fiber served as the control.The results showed that C.esculentus dietary fiber significantly promoted the production of SCFAs,but its effect was less pronounced than that of soybean dietary fiber.This study also found that high concentrations of dietary fiber significantly changed the diversity and richness of the gut microbiota,and the effects of the two dietary fibers were significantly different.C.esculentus dietary fiber significantly promoted the relative abundance of Firmicutes,Proteobacteria and Actinobacteria,but inhibited the growth of Bacteroidota.Its effect was slightly different from that of soybean dietary fiber.Moreover,compared with soybean dietary fiber,C.esculentus dietary fiber significantly had a stronger capacity to promote the growth and reproduction of Subdoligranulum,Bifidobacterium,Lactobacillus and Faecalibacterium,and significantly suppressed the growth of Prevotella and Bacteroides.This study highlights the potential of C.esculentus dietary fiber as a novel prebiotic.
作者
王玲玉
闫春梅
贾梦
黄璟
王安琪
汪轩羽
王新涛
李秀荷
王慧芳
戴福宏
李宁
周中凯
高铁成
WANG Lingyu;YAN Chunmei;JIA Meng;HUANG Jing;WANG Anqi;WANG Xuanyu;WANG Xintao;LI Xiuhe;WANG Huifang;DAI Fuhong;LI Ning;ZHOU Zhongkai;GAO Tiecheng(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Xinjiang Sanli Grain and Oil Co.Ltd.,Tumushuke 843900,China;Guangzhou Puratos Food Co.Ltd.,Guangzhou 511400,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第18期126-132,共7页
Food Science
基金
中央引导地方科技发展资金项目(22ZYCGSN00360)
天津西青重点研发项目(XQZDZX-202006)。
关键词
油莎豆
膳食纤维
体外发酵
肠道微生物
短链脂肪酸
Cyperus esculentus
dietary fiber
in vitro fermentation
gut microbiota
short-chain fatty acids
