摘要
以抑制酪氨酸酶活性为筛选指标,对5株乳酸菌发酵上清液、菌体细胞和破碎提取物的酪氨酸酶抑制活性和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率进行分析,结果表明,菌株发酵上清液抑制酪氨酸酶能力远高于菌体细胞和破碎提取物,其中MNJ-9菌株发酵上清液抑制酪氨酸酶能力可达68.9%。菌体细胞和破碎提取物仅为50.9%和34.8%。MNJ-9菌株发酵上清液对DPPH自由基清除能力可达93.1%。因此,对MNJ-9菌株进行16S rDNA菌种鉴定。基于液相色谱-质谱非靶向代谢组学对MNJ-9菌株发酵上清液进行主要成分分析。对其主要成分的10种化合物进行抑制率测定,结果表明,5-氨基戊酸抑制率最高。将5-氨基戊酸对小鼠黑色素瘤细胞(B16F10)进行细胞毒性实验、黑色素含量测定、细胞酪氨酸酶活性测定评价其美白能力。结果表明,5-氨基戊酸质量浓度为70μg/mL连续培养72 h时细胞存活率≥80%,细胞无明显毒性;质量浓度为10~70μg/mL时,细胞内黑色素含量和酪氨酸酶活性分别为85.4%~45.2%和76.53%~40.5%,说明5-氨基戊酸具有一定的美白能力。
In this study,the fermentation supernatant,cells and disrupted cell supernatant of five strains of lactic acid bacteria(LAB)were screened for tyrosinase inhibitory activity and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity.The results showed that the tyrosinase inhibitory activity of the fermentation supernatant was much higher than that of the cells and the disrupted cell supernatant.The percentage inhibition of tyrosinase activity by the fermentation supernatant of strain MNJ-9 was 68.9%compared to only 50.9%with the cells and 34.8%with the disrupted cell supernatant.The fermentation supernatant of strain MNJ-9 scavenged 93.1%of DPPH radical.Meanwhile,MNJ-9 was identified by 16S rDNA gene sequencing.The major components of the fermentation supernatant of MNJ-9 were investigated using untargeted metabolomics based on liquid chromatography-mass spectrometry(LC-MS).The tyrosinase inhibitory activity and DPPH radical scavenging capacity of 10 of the major components were measured.The results showed that 5-aminovaleric acid had the highest tyrosinase inhibitory activity and DPPH radical scavenging capacity.The cytotoxicity of 5-aminovaleric acid on mouse melanoma cells(B16F10),and its impact on the melanin content and tyrosinase activity of B16F10 cells were determined to evaluate its whitening ability.The results showed that the cell survival rate was equal to or over 80%after culture for 72 h with 70μg/mL of 5-aminovaleric acid,indicating no apparent cytotoxicity.At 5-aminovaleric acid concentrations of 10–70μg/mL,the intracellular melanin content and tyrosinase activity were 85.4%–45.2%and 76.53%–40.5%,respectively.This indicates that 5-aminovaleric acid has some whitening ability.
作者
张雪
李雪峰
胡瑞峰
郭万春
刘学军
ZHANG Xue;LI Xuefeng;HU Ruifeng;GUO Wanchun;LIU Xuejun(School of Food Science and Engineering,Jilin Agricultural University,Changchun 130000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第18期193-199,共7页
Food Science
基金
吉林省科技发展计划项目(20210202057NC)。
