摘要
目的观察氟化钠(NaF)亚慢性染毒对雄性大鼠心脏、肝、肾以及睾丸组织病理损伤及功能的影响,并探讨其可能的作用机制。方法将32只Wistar雄性大鼠随机分为对照组和低、中、高剂量NaF组,每组8只,分别予0、12、24、48 mg/kg氟化钠溶液灌胃,每周染毒6 d,持续16周。记录大鼠氟牙症发生情况,体重变化及计算大鼠心脏、肝、肾和睾丸脏器系数;ELISA法检测大鼠血清SOD、CAT、GSH-Px、MDA活力/含量水平;采用HE染色观察心脏、肝、肾和睾丸组织病理损伤;生化法检测大鼠心肌酶谱、肝功能和肾功能;并对大鼠精子质量进行分析。结果与对照组相比,低、中、高剂量NaF组大鼠上、下颌切牙均呈不同程度的氟牙症改变,提示造模成功。高剂量NaF组大鼠体重低于其余3组(P<0.05),低剂量NaF组和高剂量NaF组肾脏器系数高于对照组(P<0.05)。与对照组相比,中、高剂量NaF组CAT、GSH-Px活力均下降(P<0.05);高剂量NaF组血清中MDA含量水平升高(P<0.05);HE染色结果提示与对照组相比,低、中、高剂量NaF组心脏、肝、肾和睾丸均出现不同程度的病理性损伤;与对照组相比,各剂量NaF组LDH和CK-MB含量水平升高(P<0.05),中、高剂量NaF组大鼠血清中ALT和AST含量升高(P<0.05),高剂量NaF组大鼠血清中GGT含量升高(P<0.05),低、中、高剂量NaF组大鼠血清中BUN和Scr含量升高(P<0.05),低、中、高剂量NaF组大鼠的精子数量下降,精子活率和精子畸形率上升(P<0.05)。结论氟化钠亚慢性染毒可使大鼠多脏器组织出现损伤效应,其机制可能与氧化应激诱导有关。
Objective To investigate pathological damage and functions after subchronic exposure to sodium fluoride(NaF)on the heart,liver,kidneys,and testes of male rats,and to explore the possible mechanism.Methods Thirty-two Wistar male rats were randomly divided into control,and low,medium,and high dose groups with eight rats in each group.NaF at doses of 0,12,24,and 48 mg/kg body weight was applied by intragastric administration once a day at 6 days per week and for 16 weeks.The occurrence of dental fluorosis and body mass in each group of rats was recorded,and the organ coefficients of the heart,liver,kidneys,and testes of rats were calculated.Serum levels of SOD,CAT,GSH-Px,and MDA were measured by ELISA.Pathological changes of the heart,liver,kidneys,and testes were observed by HE staining.Biochemical method were used to determine the myocardial enzyme profile,liver functions,and kidney functions in rats,and analyze the sperm quality of rats.Results Compared with rats in the control group,rats in low,medium,and high dose groups showed different degrees of fluorosis changes in maxillary and mandibular incisors,indicating successful modeling.The body weight of rats in the high dose group was lower than that in the other three groups(P<0.05).The renal organ coefficient in low and high dose groups was higher than that in the control group(P<0.05).Compared with that in the control group,CAT and GSH-Px activity was decreased in medium and high dose groups(P<0.05).The serum level of MDA in in the high dose group was increased(P<0.05).Compared with the findings in the control group,HE staining showed different degrees of pathological damage in the heart,liver,kidneys,and testes of low,medium,and high dose groups.Compared with those in the control group,LDH and CK-MB levels were increased in each dose group(P<0.05).The serum levels of ALT and AST were increased in medium and high dose groups(P<0.05),and the serum level of GGT was increased in the high dose group(P<0.05).Serum levels of BUN and Scr were increased in low,medium,and high dose groups(P<0.05).The sperm count of rats was decreased in low,medium,and high dose groups,and sperm motility and the sperm malformation rate were increased(P<0.05).Conclusions Subchronic exposure to sodium fluoride damages multiple organs in rats,and the mechanism may be related to oxidative stress induction.
作者
黄大敏
曾维德
苏建伟
庞雅琴
郭蕊
邓树嵩
HUANG Damin;ZENG Weide;SU Jianwei;PANG Yaqin;GUO Rui;DENG Shusong(School of Public Health and Management,Youjiang Medical University for Nationalities,Baise 533000,China.;Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000)
出处
《中国比较医学杂志》
CAS
北大核心
2023年第8期8-14,74,共8页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(81660549)
广西自然科学基金(2019JJD140011,2021GXNSFAA196068)
广西高校中青年教师科研基础能力提升项目(2021KY0543)。
关键词
氟化钠
大鼠
多脏器损伤
氧化应激
sodium fluoride
rats
multiorgan damage
oxidative stress
