摘要
目的探究不同尾吊(TS)时间下失重模拟小鼠心脏功能变化。方法将30只小鼠随机分为3组:对照(CTRL)组、40 d尾吊(TS⁃40 d)组、70 d尾吊(TS⁃70 d)组。尾吊结束后,利用称重、心脏超声、苏木精⁃伊红(HE)染色和Masson染色法、实时定量PCR(qPCR)方法评价失重模拟小鼠的心脏功能。结果与CTRL组小鼠相比,TS组小鼠体重增长缓慢(P<0.01);心脏超声显示,TS⁃40 d组小鼠射血分数、缩短分数较CTRL组显著下降(P<0.05);TS⁃70 d组小鼠射血分数和缩短分数相较TS⁃40 d组显著上调。心脏形态学和质量数值显示,TS组较CTRL组心肌萎缩(P<0.0001);HE和Masson染色结果显示,TS组小鼠心脏胶原纤维呈蓝色,TS⁃70 d组更明显;qPCR结果显示,TS组较CTRL组小鼠心脏重塑因子脑尿钠肽(BNP)和纤维化标志物间质金属蛋白酶2(MMP2)表达上调,TS⁃70 d组更显著。结论TS⁃40 d小鼠心脏功能下降,心脏萎缩、纤维化;TS⁃70 d小鼠心脏功能出现适应性恢复,但出现更严重的萎缩和纤维化。
Objective To investigate the changes of cardiac functions in mice under simulated weightlessness with different durations of tail⁃suspension.Methods Thirty mice were randomly divided into three groups:the control(CTRL)group,40⁃day tail⁃suspension(TS⁃40 d)group and 70⁃day tail⁃suspension(TS⁃70 d)group.After tail suspension,the cardiac functions of mice under simulated weightlessness were evaluated by weighing,echocardiography,hematoxylin⁃eosin(HE)staining,Masson trichrome staining and real⁃time quantitative PCR(qPCR).Results Compared with the CTRL group,body weight of the TS group mice was gained more slowly(P<0.01).Echocardiography showed that the values of ejection fraction and fraction shortening of mice in the TS⁃40 d group were significantly decreased compared with the CTRL group(P<0.05),and that these values were significantly lower than those of TS⁃70 d mice.The results of cardiac morphology and mass showed that the myocardial atrophy was caused by tail⁃suspension in the TS mice compared with the CTRL mice(P<0.0001).HE and Masson trichrome staining showed that the collagen fibers in the heart of the TS group were blue,which was more obvious in the TS⁃70d group.The results of qPCR showed that the expressions of cardiac remodeling factor brain natriuretic peptide(BNP)and fibrosis marker matrix metalloproteinases⁃2(MMP2)were up⁃regulated in TS⁃40 d and TS⁃70 d groups,especially in the TS⁃70 d group.Conclusion TS⁃40 d mice show decreased cardiac function,cardiac atrophy and fibrosis while TS⁃70 d mice show adaptive recovery of cardiac function,but more severe cardiac atrophy and fibrosis.
作者
周雪梅
付业胜
张令强
ZHOU Xuemei;FU Yesheng;ZHANG Lingqiang(State Key Laboratory of Proteomics,Institute of Lifeomics,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《军事医学》
CAS
CSCD
2023年第8期603-608,614,共7页
Military Medical Sciences
基金
国家自然科学基金重大项目(82192881)
国家自然科学青年基金项目(32201023)。
关键词
失重模拟
尾吊时间
小鼠
心脏功能
simulated weightlessness
tail⁃suspension time
mice
cardiac function
