摘要
临界尺寸骨缺损的重建是组织工程中重要的挑战.胶原蛋白和羟基磷灰石复合支架因其具有良好的生物相容性、生物降解性和成骨性在骨组织工程中受到广泛关注.本研究中,我们开发了牦牛胶原蛋白-羟基磷灰石-海藻酸钠(YOHSA)三维(3D)多孔支架,对临界尺寸颅骨缺损具有良好的修复效果.以牦牛胶原蛋白(YO)为生物模板通过原位生物矿化,可形成具有良好纤维结构的纳米羟基磷灰石.YOHSA支架呈均匀的多孔结构,具有与天然骨相似的有机-无机成分,并表现出良好的生物相容性、增强的机械强度、适当的生物降解性、优异的细胞活性和显著的成骨分化能力.大鼠临界尺寸颅骨缺损模型的微型计算机断层扫描(micro-CT)和组织学染色结果均表明,YOHSA支架能显著促进骨组织的再生.这种高生物相容性和成骨诱导性的复合支架,在骨组织工程领域有广泛的应用前景.
The reconstruction of critical-size bone defects is a major challenge in tissue engineering.Collagenhydroxyapatite composite scaffolds have been extensively investigated in bone tissue engineering to their favorable biocompatibility,biodegradability,and osteogenicity.Herein,we developed a three-dimensional porous scaffold of yak bone collagen-hydroxyapatite-sodium alginate(YOHSA)with supreme healing efficacy for critical-size cranial defects.Nanofibrous hydroxyapatite with a desirable fiber structure was obtained through in-situ biomineralization utilizing yak bone collagen(YO)as a biological template.The YOHSA scaffold possessed a uniform porous structure,organicinorganic components similar to natural bone,favorable biocompatibility,enhanced mechanical strength,appropriate biodegradability,excellent cell activity,and exceptional osteogenic differentiation capability.Micro-computed tomography(micro-CT)and histological staining of a rat model of critical-size cranial defects demonstrated that the YOHSA scaffold significantly promoted bone tissue regeneration.This highly biocompatible and osteoinductive composite scaffold has broad application prospects in the field of bone tissue engineering.
作者
何会霞
王莉莉
蔡向东
李文华
肖建喜
Huixia He;Lili Wang;Xiangdong Cai;Wenhua Li;Jianxi Xiao(College of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000,China;Gansu Engineering Research Center of Medical Collagen,Lanzhou 730000,China)
出处
《中国科学：化学》
CAS
CSCD
北大核心
2023年第9期1750-1760,共11页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(编号:22074057)
甘肃省重点研发计划(编号:18YF1NA004)资助项目。
关键词
牦牛骨胶原蛋白
生物矿化
骨再生
组织工程
yak bone collagen
biomineralization
bone regeneration
tissue engineering
