摘要
本研究以汉麻分离蛋白(Hemp Protein Isolate,HPI)为原料,通过超高压辅助酶解反应对HPI进行改性,以溶解度和水解度为判定指标筛选酶解改性反应最佳条件,并探究超高压辅助酶解反应对酶解产物溶解性、起泡性、乳化性、持水性、持油性的影响。结果表明,HPI酶解反应最适条件为:加酶量(复合蛋白酶)5000 U/g、酶解改性pH8.0、酶解改性温度55℃、酶解改性时间50 min。以HPI为对照,当压力为200 MPa时,酶解产物的溶解度、起泡性、乳化性、持油性最高,压力为100 MPa时,泡沫稳定性最好,酶解后的乳化稳定性存在不同程度的下降,压力为0.1 MPa时其持水性达到最大值。综上所述,超高压技术能够有效促进HPI的酶解改性反应,且压力为200 MPa时,酶解产物的理化性质最好。
Hemp protein isolate(HPI)was used as raw material to modify HPI through ultra-high pressure assisted enzymatic hydrolysis reaction.The solubility and degree of hydrolysis were used as criteria to screen the optimal conditions for the enzymatic hydrolysis modification.The influence of ultra-high pressure assisted enzymatic hydrolysis on solubility,foaming,emulsification,water retention and oil retention of enzymatic hydrolysis products was explored.The results showed that the optimum conditions for HPI enzymatic reaction were as follows:Adding amount of enzyme(complex protease)5000 U/g,pH8.0,temperature 55℃and time 50 min.Taking HPI as the control,when the pressure was 200 MPa,the solubility,foaming property,emulsification property and oil holding capacity of enzymatic hydrolysis products were the highest,when the pressure was 100 MPa,the foaming stability was the best,the emulsification stability after enzymatic hydrolysis had different degrees of decline,when the pressure was 0.1 MPa,the water holding capacity reached the maximum.In conclusion,the ultrahigh pressure technology can effectively promote the enzymatic hydrolysis modification reaction of HPI,and the physical and chemical properties of the enzymatic hydrolysis products were the best when the pressure is 200 MPa.
作者
刘容旭
李春雨
王语聪
谢智鑫
谢宜桐
李双鹏
刘丹怡
韩建春
LIU Rongxu;LI Chunyu;WANG Yucong;XIE Zhixin;XIE Yitong;LI Shuangpeng;LIU Danyi;HAN Jianchun(Heilongjiang Green Food Science Research Institute,Harbin 150028,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China)
出处
《食品工业科技》
CAS
北大核心
2023年第19期99-107,共9页
Science and Technology of Food Industry
基金
降三高系列功能性产品研发项目(lyy20200921)。
关键词
汉麻分离蛋白(HPI)
超高压(UHP)
酶解
理化性质
hemp protein isolate(HPI)
ultra-high pressure processing(UHP)
enzymatic hydrolysis
physicochemical property
