摘要
目的研究三七三醇皂苷主要成分对剪切力诱导的血小板活化和聚集的影响。方法建立狭窄微流控芯片模型,采用微流控芯片技术评价在不同浓度(0.05、0.1、0.25、0.5 mg/mL)及不同剪切率(1500、4500、9000 s-1)条件下Rg1、Re和R1对剪切力诱导的血小板聚集影响;采用流式细胞术定量分析Rg1、Re和R1对高剪切力诱导的血小板活化(P-选择素和PAC-1)的影响;采用PL-12分析仪检测Rg1、Re和R1对AA和ADP诱导的血小板聚集的影响。结果Rg1、Re和R1呈浓度依赖性抑制高剪切力诱导的血小板聚集(P<0.01),而对较低剪切力无抑制作用,其中Re抑制作用最强,三者的IC50分别为0.1、0.07、0.2 mg/mL,当3种化合物在相同浓度下联合使用时没有观察到协同作用。Rg1、Re和R1抑制P-选择素和PAC-1的表达(P<0.01),抑制效果与聚集实验结果一致。Rg1、Re和R1能够抑制AA和ADP诱导的血小板聚集(P<0.05)。结论三七三醇皂苷主要成分通过抑制剪切力诱导的血小板聚集发挥新的抗血小板活性。
Objective To determine the effects of main components(Rg1,Re and R1)of Panax notoginseng saponins on platelet activation and aggregation induced by shear stress.Methods A narrow microfluidic chip model was established,and used to evaluate the effects of Rg1,Re and R1 at diferent concentrations(0.05,0.1,0.25 and 0.5 mg/mL)on shear-induced platelet aggregation under diferent shear ates(1500,4500 and 9000s^(-1)).Flow cytometry was used to quantitatively detect the shear-induced platelet activation(P-selectin and PAC-1)after the treatment of Rg1,Re and R1.PL-12 analyzer was performed to measure the effects of Rg1,Re and R1 on platelet aggregation induced by arachidonic acid(AA)and adenosine diphosphate(ADP).Results Rg1,Re and R1 inhibited platelet aggregation induced by high shear stress in a concentration-dependent manner(P<0.01),but had no such inhibitory effect under lower shear stress.Among the 3 components,Re had the strongest inhibitory effect,and their ICg0 value was 0.1,0.07,and 0.2 mg/mL,respectively.No synergistic effect was observed when the 3 components were used together at the same dose.Rg1,Re and R1 inhibited the expression of P-selectin and PAC-1(P<0.01),which was consistent with the results of the aggregation experiment.In addition,the 3 components also could inhibit platelet aggregation induced by AA and ADP.Conclusion The main components of Panax notoginseng saponins exert their antiplatelet activity by inhibiting shear-induced platelet aggregation.
作者
黄小静
张天聪
高雪梅
宦宣容
李远
HUANG Xiaojing;ZHANG Tiancong;GAO Xuemei;HUAN Xuanrong;LI Yuan(Clinical Laboratory Centre,Yongchuan Hospital of Chongqing Medical University,Chongqing,402160,China)
出处
《陆军军医大学学报》
CAS
CSCD
北大核心
2023年第19期2037-2044,共8页
Journal of Army Medical University
基金
国家自然科学基金青年科学基金(11702047)
重庆市科卫联合医学科研项目中青年高端人才项目(2023GDRC008)
重庆医科大学附属永川医院研究生创新基金项目(YJSCX202202)。
