摘要
为筛选出耐盐性较强的加工番茄种质,以石河子大学农学院提供的50份加工番茄种质资源为材料,采用双层滤纸培养法将种子在90 mmol·L^(-1) NaCl条件下进行耐盐性鉴定,以蒸馏水处理作为对照,分别测定发芽势、发芽率、胚轴长、胚根长、发芽指数、活力指数,并计算其盐胁迫相对指数,利用相关性分析法、主成分分析法、隶属函数法及聚类分析法进行综合评价。结果表明,盐胁迫下,6个萌发指标均受到不同程度的抑制,50份加工番茄种质在种子萌发期耐盐性有显著差异;通过主成分分析可将多个萌发指标降维至2个综合因子,累计方差贡献率达到89.149%;通过隶属函数法和聚类分析可将50份加工番茄种质划分为高度耐盐型(18份)、中度耐盐型(24份)和盐敏感型(8份)3类。研究结果可为盐渍化地区选育耐盐加工番茄新品种提供育种材料。
In order to screen out the processed tomato germplasm with strong salt tolerance,50 processed tomato germ-plasm resources provided by the College of Agriculture,Shihezi University were used as materials,and the salt tolerance of seeds was identified under the condition of 90 mmol·L^(-1) NaCl by double filter paper culture method.Moreover,the dis-tilled water treatment as a control was used instead of the NaCl treatment.The germination potential,germination rate,cotyl length,radicle length,germination index,and vigor index of the processed tomato seeds were determined and salt stress relative index was calculated.In addition,the correlation analysis,principal component analysis,membership func-tion method,and cluster analysis method were used for comprehensive evaluation.The results suggested that the six ger-mination indexes were inhibited to different degrees under salt stress,and the salt tolerance of 50 tomato germplasm showed significant differences during seed germination.Through principal component analysis,multiple germination in-dexes could be reduced to two comprehensive factors,and the cumulative variance contribution rate reached 89.149%,rel-ative germination potential,relative germination index and relative vigor index of the tomato seeds as the main indexes were used to identify the salt tolerance of processed tomato at germination stage.In addition,the 50 tomato germplasm were divided into the high salt tolerance type(18),the medium salt tolerance type(24),and the salt sensitive type(8)ac-cording to membership function method and cluster analysis.The results can provide breeding materials for breeding new varieties of salt-tolerant processed tomatoes in salinized areas.
作者
张明亚
庞胜群
刘玉东
吉雪花
杨岚
胡继红
ZHANG Mingya;PANG Shengqun;LIU Yudong;JI Xuehua;YANG Lan;HU Jihong(Key Laboratory of Characteristic Physiology and Germplasm Resources of Fruits and Vegetables/College of Agriculture,Shihezi University,Shihezi 832003,Xinjiang,China)
出处
《中国瓜菜》
CAS
北大核心
2023年第10期84-90,共7页
China Cucurbits And Vegetables
基金
石河子大学新品种培育专项(YZZX202204)
国家自然科学基金(32160713)。
关键词
加工番茄
种子萌发期
耐盐性
综合评价
Processing tomato
Seed germination stage
Salt tolerance
Comprehensive evaluation
