摘要
基于酪蛋白的活性物质递送体具有多种优良性能,为提高酪蛋白(CN)胶束对亲水性物质的负载率,在胶束纳米结构尺度研究了胶束扰动因素对溶菌酶(Ly)负载的影响,制备了具有核壳结构的抑菌纳米粒。粒径分布和Zeta电位表明,低浓度NaCl和乙二胺四乙酸(EDTA)有利于CN胶束的部分解离,使Ly负载率提高了8%;红外分析显示,不同胶束扰动因素下复合纳米粒中蛋白质的二级结构和氢键缔合发生了改变;扫描电镜表明CN-Ly纳米粒具有球形结构。低分子量壳聚糖包覆纳米粒后,对金黄色葡萄球菌(S.aureus)和大肠杆菌(E.coli)均具有较强的抑菌活性,且该复合纳米粒在pH5.4的酸环境中具有缓释性能,24 h释放率达到61.5%,活性保留率达到80%以上。该纳米粒在食品和医药工业有着潜在的应用前景。
Casein-based active substance deliverers have a variety of excellent properties.In order to improve the encapsulation efficiency of hydrophilic substances on casein(CN)micelles,the effect of micelle disturbance factors on lysozyme(Ly)loading efficiency was studied at the nanoscale of CN micelles,and the bacteriostatic nanoparticles with core-shell structure were prepared.The particle size distribution and Zeta potential indicate that low concentrations of NaCl and EDTA favor the partial dissociation of CN micelles and increase the encapsulation efficiency of Ly by 8%.Fourier transform infrared spectroscopy(FT-IR)results show that the protein’s secondary structure and hydrogen bond association of composite nanoparticles change under different micellar disturbance factors,and SEM images show that CN-Ly nanoparticles display spherical structure.The nanoparticles coated with the low molecular weight chitosan have a high inhibitory activity against both S.aureus and E.coli.In addition,the CN-Ly-CTS composite nanoparticles show controlled release performance in acid environment of pH5.4 with a 24 h-release rate of 61.5%,and the enzyme activity retention rate remains more than 80%.The nanoparticles have potential applications in food and pharmaceutical industries.
作者
贺宝元
张思冉
李欣雨
张磊
周妍
薛海燕
Baoyuan He;Siran Zhang;Xinyu Li;Lei Zhang;Yan Zhou;Haiyan Xue(College of Bioresources Chemical&Materials Engineering,Shaanxi University of Science&Technology;School of Biological and Pharmaceutical Sciences,Shaanxi University of Science&Technology,Xi′an 710021,China)
出处
《高分子材料科学与工程》
EI
CAS
CSCD
北大核心
2023年第8期32-40,共9页
Polymer Materials Science & Engineering
基金
陕西省科技计划(2022GY-361,2022KXJ-011)
陕西省重点研发计划(2020ZDLNY02-09)
陕西科技大学博士科研启动基金项目(2023BJ-20)
西安市科技计划农业科技创新工程(20NYYF0016)。
关键词
酪蛋白胶束
结构变换
溶菌酶
壳聚糖
抑菌纳米粒
casein micelles
structure transformation
lysozyme
chitosan
bacteriostatic nanoparticles
