摘要
目的探讨肺炎支原体社区获得性呼吸窘迫综合征(CARDS)毒素对小鼠外周血单核细胞亚群的影响。方法制备重组CARDS毒素。32只小鼠按随机数字表分为模型组和正常对照组,每组16只。模型组每只小鼠气管注射40μL含有50μg CARDS毒素的PBS,正常对照组气管注射同等剂量的PBS。造模后24 h腹主动脉采血,流式细胞术检测外周血单核细胞亚群(CD11b+Ly6G-Ly6C+)比例变化;采用HE染色观察肺组织病理学变化,进行肺组织损伤评分;Real-time PCR检测肺组织中白细胞介素(IL)-1β和IL-18表达水平;采用酶联免疫吸附试验检测外周血IL-1β和IL-18水平。结果与正常对照组相比,模型组小鼠单核细胞CD11b+Ly6G-Ly6C+比例升高(P<0.05);HE染色可见模型组肺泡壁增厚、炎性细胞浸润增加,病理评分显著增高(P<0.01);肺组织及血清炎性因子IL-1β和IL-18表达水平均增高(P<0.01)。结论CARDS毒素诱导的小鼠中Ly6C+单核细胞占比升高,其可能与肺炎支原体肺炎的发病密切相关。
Objective To investigate the effect of Mycoplasma pneumoniae community-acquired respiratory distress syndrome(CARDS)toxin on peripheral blood mononuclear cell subsets in mice.Methods The recombinant CARDS toxin was prepared.Thirty-two mice were divided into the model group and the normal control group according to random number table with 16 mice in each group.Each mouse in the model group was injected with 40μL PBS containing 50μg of CARDS toxin.In the normal control group,the same dose of PBS was injected into trachea.Blood samples were collected from abdominal aorta 24 h after the modeling,and the proportion of peripheral monocyte subsets(CD11b+Ly6G-Ly6C+)was detected by flow cytometry.The mice were sacrificed,and lung histopathological changes were observed by HE staining,and the lung tissue injury score was performed.The expression levels of interleukin(IL)-1βand IL-18 in lung tissue were detected by Real-time PCR.The levels of IL-1βand IL-18 in peripheral blood were determined by enzyme-linked immunosorbent assay.Results Compared with the normal control group,CD11b+Ly6G-Ly6C+ratio of mouse monocytes was increased in the model group(P<0.05).HE staining showed that alveolar wall thickened,inflammatory cell infiltration increased,pathological score increased significantly(P<0.01),the expression levels of IL-1βand IL-18 mRNA in lung tissue and contents of inflammatory cytokines IL-1βand IL-18 in serum increased significantly(P<0.01).Conclusion The increased proportion of Ly6C+monocytes in mice induced by CARDS toxin may be closely related to the incidence of Mycoplasma pneumoniae pneumonia.
作者
张岑
王志华
杨磊
ZHANG Cen;WANG Zhihua;YANG Lei(Department of Critical Care,Tianjin Nankai Hospital,Tianjin 300100,China)
出处
《天津医药》
CAS
北大核心
2023年第10期1080-1084,共5页
Tianjin Medical Journal
基金
天津市卫生健康委员会中西医结合科研课题(2021094)。
