摘要
【目的】筛选多态性高、通用性强等的SSR位点,评估筛选所得SSR位点的组合在山茶属品种中的鉴别能力;在保持最大鉴别能力的基础上,确定可用于山茶属品种鉴别所需的最少SSR位点的组合,提高品种鉴别效率。【方法】本研究以111份山茶属品种为材料,结合凝胶电泳、荧光毛细管电泳、位点多态性分析和位点组合品种鉴别力(VDP)分析等方法,筛选评价SSR位点并对SSR位点组合的品种鉴别力进行分析与评价。【结果】本研究筛选获得17个SSR位点,占筛选位点总数的30.9%,在111份山茶属品种中,共检测到166个等位基因,单位点基因型数量范围为6~35,均值为23.353个,观测等位基因数(Na)范围为3~15,均值为9.765个,多态性信息含量(PIC)的范围为0.447~0.835,均值为0.696,位点缺失率的范围为0~4.50%,均值为0.90%。当差异位点数=1时,17个SSR位点在全部品种(系)、红山茶组品种和金花茶组无性系中达到的R-VDP最大值均为1,可将SSR位点数量分别缩减到11、10、5个,分别占筛选所得位点数的64.7%,58.8%和29.4%;当差异位点数=2时,17个SSR位点在全部品种(系)、红山茶组品种和金花茶组无性系中达到的R-VDP最大值分别为0.964,0.952和1.000,可将SSR位点数量分别缩减到12、12、和7个,分别占筛选所得位点数的70.6%、70.6%和41.2%;当差异位点数=3时,17个SSR位点在全部品种(系)、红山茶组品种和金花茶组无性系中达到的R-VDP最大值分别为0.946,0.929,1.000,可将SSR位点数量分别缩减到8、7和8个,分别占筛选所得位点数的47.1%、41.2%和47.1%。当差异位点数≤6时,17个SSR位点组合对山茶属测试品种(系)群的RVDP最大值可保持相对稳定的水平。【结论】本研究筛选验证获得17个多态性高、通用性强等的SSR位点;这些位点的组合在山茶属品种(系)群中均表现出良好的品种鉴别能力;确定了山茶属不同类群品种(系)鉴别所需的最少SSR位点的组合,构建了一套山茶属SSR位点组合品种鉴别能力分析评价的体系,为山茶属品种鉴别及相关标准的制定等奠定了基础。
【Objective】The paper aims to screen SSR loci with high polymorphism and strong universality in Camellia,evaluate the variety discrimination power(VDP)of different SSR loci combinations and determine the minimum numbers of SSR loci in combination for variety discrimination at different Camellia groups,so as to improve the efficiency of variety discrimination in practice.【Method】In this study,a total of 111 Camellia varieties were used to screen and evaluate SSR loci and the variety discrimination power of combinations of SSR loci by combining the methods of gel electrophoresis,fluorescent capillary electrophoresis,loci polymorphism analysis and loci combination variety discrimination power(VDP)analysis.【Result】A total of 17 SSR loci were screened and obtained,accounting for 30.9%of the total number of selected loci.In 111 Camellia varieties,166 alleles were detected with the 17 SSR loci.The number of genotypes per locus ranged from 6 to 35,with an average of 23.353.The observed number of alleles(Na)ranged from 3 to 15,with a mean of 9.765.The polymorphic information content(PIC)ranged from 0.447 to 0.835,with a mean of 0.696.The range of missing data rate of loci was 0–4.50%,with a mean of 0.90%.When the number of differential loci=1,the maximum R-VDP of 17 SSR loci in all varieties(clones),Sect.Camellia varieties and Sect.Chrysantha clones were all 1,and the number of SSR loci was able to be reduced to 11,10 and 5,respectively accounting for 64.7%,58.8%and 29.4%of the screened loci in the different groups.When the number of differential loci=2,the maximum RVDP values of 17 SSR loci in all varieties(clones),Sect.Camellia varieties and Sect.Chrysantha clones were 0.964,0.952 and 1.000,respectively,and the number of SSR loci was able to be reduced to 12,12 and 7,respectively accounting for 70.6%,70.6%and 41.2%of the screened loci in the different groups.When the number of differential loci=3,the maximum R-VDP values of 17 SSR loci in all varieties(clones),Sect.Camellia varieties and Sect.Chrysantha clones were 0.946,0.929,and 1.000,respectively,and the number of SSR loci was able to be reduced to 8,7 and 8,respectively,accounting for 47.1%,41.2%and 47.1%of the screened loci in the different groups.When the number of differential loci was≤6,the maximum R-VDP of 17 SSR loci combinations for the tested varieties(clones)of Camellia could maintain a relatively stable level.【Conclusion】In this study,17 SSR loci with high polymorphism and strong universality have been screened and verified.The combinations of these loci show well variety discrimination power in the tested Camellia groups.The minimum numbers of SSR loci in combinations for variety discrimination have been determined in different variety(clones)groups of Camellia,and a set of analyses and evaluation system for variety discrimination power of combinations of SSR loci in Camellia have been constructed.These results provide useful information and tools for variety identification and development of related standards.
作者
李丰钰
黄平
郑勇奇
李长红
张雨婷
薛克娜
宗亦臣
赵鸿杰
Li Fengyu;Huang Ping;Zheng Yongqi;Li Changhong;Zhang Yuting;Xue Kena;Zong Yichen;Zhao Hongjie(College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109;Laboratory of Molecular Testing for New Plant Varieties of National Forestry and Grassland AdministrationKey Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration Research Institute of Forestry Chinese Academy of Forestry National Key Laboratory of Tree Genetics and Breeding,Beijing 100091;Foshan Institute of Forestry,Foshan 528234)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2023年第8期74-84,共11页
Scientia Silvae Sinicae
基金
国家林业与草原种质资源库(2005DKA21003)。
