摘要
以聚乙二醇化石墨烯量子点标记的适配体(GQDs-PEG-Apt)作为甲胎蛋白(AFP)的特异性识别分子和能量供体,以WS_(2)/Fe_(3)O_(4)纳米复合物为单一能量受体,构建磁分离适配体荧光传感器并用血清AFP定量检测。样本中没有AFP时,能量供体与受体间π-π堆积作用和非辐射共振能力转移(FRET)令GQDs-PEG-Apt荧光猝灭;存在AFP时,GQDs-PEG-Apt与靶标结合并从WS_(2)/Fe_(3)O_(4)纳米复合物表面脱离,体系荧光强度得以恢复;经过磁性分离后,传感体系上清液487 nm处荧光强度随AFP浓度增大而增强;浓度在5~1000 ng/mL范围内,传感体系荧光相对恢复值与AFP浓度呈现良好的线性关系,线性相关系数为0.989。构建的传感器具有良好的特异性,可实现血清AFP定量检测,其检测限(LOD)为0.5 pg/mL,相对标准偏差(RSD)为3.32%~6.41%,回收率为91.92%~99.28%。
A magnetic separation aptamer fluorescence sensor was constructed and quantitatively detected with serum AFP by using aptamer labeled with polyethylene glycol fossil graphene quantum dots(GQDs-PEG-Apt)as the specific recog-nition molecule and energy donor of alpha-fetoprotein(AFP)and WS_(2)/Fe_(3)O_(4) nanocomposite as a single energy receptor.When there is no AFP in the sample,theπ-πstacking interaction between the energy donor and the receptor and Förster resonance energy transfer(FRET)makes the fluorescence of GQDs-PEG-Apt quench.When there is AFP in the sample,GQDs-PEG-Apt binds to the target and breaks away from the WS_(2)/Fe_(3)O_(4)nanocomposite,and the fluorescence of the system can be restored.After magnetic separation,the fluorescence intensity at 487 nm in the supernatant of the sensor system increased with the increase of the concentration of AFP.In the concentration range of 5~1000 ng/mL,the relative recovery rate of fluorescence of the system has a good linear relationship with AFP concentration,and the linear correlation coefficient is 0.989.The sensor constructed in this paper has good specificity and can realize the quantitative detection of serum AFP.Its limit of detection(LOD)is 0.5 pg/mL,relative standard deviation(RSD)is 3.32%~6.41%,and recovery rate is 91.92%~99.28%.
作者
李肖沙
刘莹
申炳俊
金丽虹
夏冰
LI Xiaosha;LIU Ying;SHEN Bingjun;JIN Lihong;XIA Bing(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022;Hangzhou Clongene Biotech Co.,Ltd,Hangzhou 311121)
出处
《长春理工大学学报（自然科学版）》
2023年第5期122-129,共8页
Journal of Changchun University of Science and Technology(Natural Science Edition)
基金
吉林省科技厅发展计划项目(20210204194YY)
吉林省教育厅科学技术研究项目(JJKH20220782KJ)。
