摘要
目的:分析血管性痴呆患者的肠道菌群丰度特征,探讨肠道菌群失调与血管性痴呆(VaD)的相关性。方法:收集2019-01~2019-11新疆医科大学附属中医医院脑病科TOAST分型为大动脉粥样硬化性脑梗死后所致的血管性痴呆(VaD)患者30例为VaD组,并收集同期于本院健康体检年龄、性别相匹配的认知功能正常者30名作为对照组。采集所有研究对象的粪便,提取DNA,质检合格后应用16SrRNA高通量测序技术检测粪便中的微生物群组成,进行两组之间肠道菌群丰度分析,比较其差异性。结果:(1)OTUs(Operational Taxonomic Units,OTUs)序列共注释31个菌属,与对照组比较,VaD组拟杆菌、乳杆菌、大肠埃希氏菌丰度较高;对照组中双歧杆菌、链球菌丰度较高,两组的菌群丰度具有差异性(P<0.05)。(2)通过样本群落结构分布显示VaD组中拟杆菌、乳杆菌、大肠埃希氏菌丰度较高;对照组中双歧杆菌、罗氏菌、劳特氏菌丰度较高;克雷伯氏菌、肠道巴恩斯氏菌、小杆菌、柯林斯氏菌丰度在两组中均偏低,对照组低于VaD组,差异有统计学意义。(3)通过boxplot分析显示,在属水平上,VaD组的肠道菌群的丰富度低于对照组;劳特氏菌属(P=0.032)、毛螺菌(P=0.002)、瘤胃菌(P=0.001)在对照组中丰度较高;志贺氏菌属(P=0.001)、克雷伯氏菌(P=0.014)、普氏菌(P=0.02)在VaD组中丰度较高;(4)Lefse分析显示变形菌门、琥珀酸弧菌科、肠杆菌科、志贺氏杆菌属在VaD组中丰度较高,为优势菌属;韦荣球菌科、劳特氏菌属、普雷沃菌属、毛螺菌科等在对照组中丰度较高,为优势菌属。结论:血管性痴呆患者存在肠道菌群丰度改变,可能出现肠道菌群失调,推测肠道菌群及其代谢产物参与血管性痴呆的病理进程及发生发展。
Objective:To analyze the characteristics of intestinal flora abundance in patients with vascular dementia,and to explore the correlation between intestinal flora imbalance and vascular dementia(VaD).Methods:From January 2019 to November 2019,30 cases of VaD patients caused by large atherosclerotic cerebral infarction were collected from the TOAST classification of the Department of Brain Diseases.30 patients with normal cognitive function matched by age and gender were used as control group.The feces of all research subjects were collected,DNA was extracted,and 16SrRNA high-throughput sequencing technology was used to detect the composition of the microbiota in the feces after passing the quality inspection,and the abundance of intestinal flora between the two groups was analyzed to compare their differences.Results:(1)A total of 31 bacterial genera were annotated with Operational Taxonomic Units(OTUs)sequences.Compared with the control group,Bacteroides,Lactobacillus,Escherichia-Shigella were abundant in the VaD group.The abundance of Bifidobacterium and Streptococcus is higher in the control group,and the abundance of the two groups of bacteria is different(P<0.05).(2)The distribution of sample community structure showed that the abundance of Bacteroides,Lactobacillus,Escherichia coli was higher in the VaD group;Bifidobacterium,Roseburia in the control group,Routerella are more abundant;Klebsiella,Barnesiella,Microbacteria,Collinsella are abundant,The degree is low in the two groups,the control group is lower than the VaD group,the difference is statistically significant.(3)Through boxplot analysis,at the genus level,the abundance of the intestinal flora of the VaD group is lower than that of the control group;Blautia(P=0.032),Lachnos piraceae_NK4A136_group(P=0.002),Ruminococcaceae_UCG−014(P=0.001)in the control group,the abundance is higher;Escherichia-Shigella(P=0.001),Klebsiella(P=0.014),Prevotella_9(P=0.02)have higher abundance in VaD group;(4)Lefse analysis shows Proteobacteria,Succinivibrionaceae,Enterobacteriaceae,Shigella are more abundant in VaD group and are the dominant genus;Veronococcus,Lauterella,Prevotella In the control group,the abundance of spirulina and Lacetospirillaceae is the dominant genus.Conclusion:There are changes in the abundance of intestinal flora in patients with vascular dementia,and intestinal flora may be imbalanced.It is speculated that the intestinal flora and its metabolites are involved in the pathological process and development of vascular dementia.
作者
徐梦
刘艳梅
李丹
张玉洁
张燕
许奔奔
郭晨晨
麦尔哈巴·赛里木
孟新玲
Meng XU;Yanmei LIU;Dan LI;Yujie ZHANG;Yan ZHANG;Benben XU;Chenchen GUO;Maierhaba sailimu;Xinling MENG(Department of Neurology,Traditional Chinese Medicine Hospital of Xinjiang Uygur Sailimu Autonomous Region Urumqi 830000,China)
出处
《阿尔茨海默病及相关病杂志》
2023年第3期206-214,共9页
Chinese Journal of Alzheimer's Disease and Related Disorders
基金
新疆维吾尔自治区自然科学基金资助项目(2022D01C161)
新疆医科大学附属中医医院院级课题(ZYY2020ZD01)。
