摘要
通过筛选可降解类胡萝卜素的烟草内生菌,并应用于国产雪茄烟叶发酵从而实现烟叶香韵和感官质量提升。首先,原位培养基中添加烟叶粉末模拟原位生长环境以富集烟叶内生菌,结合流式分选技术,从优质雪茄烟叶内生菌中筛选具有β-胡萝卜素降解能力的菌株;将菌株接种至国产雪茄烟叶发酵,检测发酵烟叶类胡萝卜素降解产物含量并进行感官评价。结果显示,共筛选获得21株内生菌对β-胡萝卜素的降解率超过50%,主要为农杆菌属、根瘤菌属、鞘氨醇杆菌属、肠杆菌属等。菌株C31、C11、H4能够增加烟叶香韵类型,明显改善烟叶香气质、香气量、杂气、刺激性等指标,发酵烟叶类胡萝卜素降解产物含量分别是对照的1.38、1.28、1.43倍,其中法尼基丙酮、香叶基丙酮、巨豆三烯酮的增加强化了烟叶焦甜香、花香、木香香韵。综上,原位筛选获得的3株类胡萝卜素降解内生菌C31、C11、H4能够较好地改善国产雪茄烟叶品质,可作为新型雪茄工业微生物制剂和特征香韵雪茄微生物制剂进一步开发应用。
Endophyte degrading carotenoids were screened and applied to domestic cigar leaves to increase domestic cigar tobacco leaves(CTLs)aroma and improve sensory quality.CTLs powder was added into in situ medium to simulate the growth environment of tobacco endophytes,and enrichment culture combined with flow cytometry technology was used to screen strains withβ-carotene-degrading ability from high-quality cigar tobacco endophytes.The strains inoculated into domestic CTLs,and the contents of carotenoid degradation products were detected and the sensory quality of CTLs was analyzed.Total 21 endophytes hadβ-carotene degradation rate over 50%,mainly including Agrobacterium,Rhizobium,Sphingobacterium,Enterobacter,etc.These strains made different effects on CTLs sensory quality,among which strains C31,C11 and H4 increased the aroma type,significantly improved the aroma quality,aroma amount,miscellaneous gas,irritation and other CTLs sensory index.The carotenoid-degrading product content from strain C31,C11 and H4 CTLs was 1.38,1.28 and 1.43 times compared with CK,respectively.The farnesyl acetone,geranyl acetone and megastigmatrienone strengthened the burnt sweet,flowery and woody aroma of CTLs.The three endophytes C31,C11 and H4 obtained by in situ screening can better improve the domestic CTLs sensory quality,and can be further developed and applied as microbial preparations of new cigar industry and characteristic aromatic cigar.
作者
吴巧茵
施友志
李林林
彭政
谭再钰
刘利平
张娟
潘勇
WU Qiao-yin;SHI You-zhi;LI Lin-lin;PENG Zheng;TAN Zai-yu;LIU Li-ping;ZHANG Juan;PAN Yong(Key Laboratory of Industrial Biotechnology of Ministry of Education,Jiangnan University,Wuxi 214122;School of Biotechnology,Jiangnan University,Wuxi 214122;Science Center for Future Foods,Jiangnan University,Wuxi 214122;China Tobacco Hubei Industry Co.,Ltd.,Wuhan 430040)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第9期192-201,共10页
Biotechnology Bulletin
基金
国家重点研发计划(2019YFC1605800)。
关键词
类胡萝卜素
降解
内生菌
原位筛选
雪茄烟叶
发酵
提质
增香
carotenoids
degradation
endophyte
situ screening
cigar tobacco leaves
fermentation
quality improvement
aroma enhancement
