摘要
该试验旨在评价中草药提取物及发酵中草药对大口黑鲈生长性能、血清抗氧化能力及肝脏健康的影响。选用初始均重为4.40 g左右的幼鱼840尾,随机分为6组,每组4个重复,每个重复35尾。在基础饲料中分别添加0、0.05%、0.1%、0.2%、0.4%的中草药提取物以及0.4%的发酵中草药,配置6种等氮等能的试验饲料,分别记为G0、G0.05、G0.1、G0.2、G0.4、GF0.4,试验周期为51 d。结果显示:各组间大口黑鲈末均重、增重率、特定生长率、存活率和摄食率差异不显著(P>0.05)。与G0组比较,G0.1组血清谷丙转氨酶、谷草转氨酶的活性显著升高(P<0.05),G0.05组血清谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性显著升高(P<0.05),G0.1和GF0.4血清丙二醛(MDA)的含量显著降低(P<0.05)。肝脏组织切片显示,G0组胞质疏松淡染,少量淋巴细胞灶性浸润,G0.05、G0.1、G0.2组偶见小灶性淋巴细胞浸润;G0.4组可见多量肝细胞水样变性,胞质疏松淡染,少量淋巴细胞浸润;而GF0.4组未见明显异常。综上所述,低鱼粉饲料中添加中草药提取物和发酵中草药对大口黑鲈生长性能影响不显著,但添加0.05%中草药提取物以及0.4%发酵中草药能够提高低鱼粉养殖大口黑鲈的抗氧化能力和肝脏组织健康状况。
This experiment was conducted to evaluate the effects of Chinese herbal preparations on growth performance,serum antioxidant capacity and liver health of largemouth bass.A total of 840 juvenile fish with an average initial weight of about 4.40 g were randomly divided into 6 groups with 4 replicates per group and 35 fish per replicate.The basic diet was supplemented with 0,0.05%,0.1%,0.2%,0.4%Chinese herbal extracts and 0.4%fermented Chinese herbal,respectively.Six isonitrogenous and isoenergetic experimental diets were recorded as G0,G0.05,G0.1,G0.2,G0.4 and GF0.4,respectively.The experiment was lasted 51 days.The results showed,there were no significant differences in average weight,weight gain rate,specific growth rate,survival rate and feed intake of largemouth bass among all groups(P>0.05).Compared with G0 group,the VSI and HSI in G0.05 group and HSI in GF0.4 group were the lowest,but the differences were not significant(P>0.05).The activities of AST and ALT in G0.1 group,the activities of GPX,SOD and CAT in G0.05 group were significantly increased(P<0.05),while the MDA content in G0.1 and GF0.4 was significantly decreased(P<0.05).The liver tissue sections showed that the cytoplasm of G0 group was loose and lightly stained,and a few lymphocytes were focal infiltration,while G0.05,G0.1 and G0.2 groups only occasionally showed small focal lymphocytes infiltration.G0.4 group showed more watery degeneration of hepatocytes,loose cytoplasm and light staining,and a small amount of lymphocyte infiltration.No obvious abnormality was observed in GF0.4 group.In conclusion,supplementation of Chinese herbal extract and fermented Chinese herbal in low fish meal diet has no significant effect on the growth performance of largemouth bass,but supplementation of 0.05%Chinese herbal extract and 0.4%fermented Chinese herbal can improve the antioxidant capacity and liver tissue health of largemouth bass which fed low fish meal diet.
作者
胡俊茹
陈晓瑛
吴浩敏
邓敬明
袁名贵
HU Junru;CHEN Xiaoying;WU Haomin;DENG Jingming;YUAN Minggui(Guangdong Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;Guangzhou Danong Biotechnology Co.LTD,Guangzhou Guangdong 510640;Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640)
出处
《广东畜牧兽医科技》
2023年第5期39-45,共7页
Guangdong Journal of Animal and Veterinary Science
基金
广东省企业科技特派员专项(GDKTP2021047100)。
关键词
中草药制剂
大口黑鲈
生长性能
抗氧化能力
肝脏组织结构
Chinese herbal
Largemouth bass
Growth performance
Antioxidant capacity
Liver tissue structure
