摘要
为研究双黄连口服液制剂醇沉物的乳酸菌液态发酵工艺,首先确定双黄连口服液制剂醇沉物添加量,再以活菌数作为指标,通过单因素试验分别研究接种量、发酵时间和发酵温度的影响,并采用响应面法优选最佳发酵条件,最后进行平行放大试验验证。结果:含20%的醇沉物、接种量4%、发酵温度32.7℃、发酵时间26 h时活菌数可达3.03×10^(9)CFU/mL,为最佳发酵条件。平行放大验证试验表明,该发酵工艺可用于制备双黄连口服液醇沉物乳酸菌液态发酵制剂。
To study the liquid fermentation process of the alcohol sediment of Shuanghuanglian oral liquid(SHL·AS)with lactic acid bacteria,the quantity of live bacteria was used as an evaluation indicator.The amount of SHL·AS added was first determined,and then the effects of inoculation amount,time duration,and temperature on the fermentation process were studied through single factor experiments.The response surface technology was used to optimize the fermentation conditions,and parallel experiments were conducted to verify the results.The results showed the optimal fermentation conditions to be 20%SHL·AS with an inoculation amount of 4%,a temperature of 32.7℃,and a duration of 26 hours,under which the quantity of viable bacteria was as high as 3.03×10^(9)CFU/mL.The parallel validation test results confirmed that these conditions were optimal.This fermentation process might be used to produce SHL·AS lactic acid bacteria liquid fermentation preparation.
作者
王清龙
王坤丽
赵圣振
袁琦昱
汤法银
WANG Qinglong;WANG Kunli;ZHAO Shengzhen;YUAN Qiyu;TANG Fayin(Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Henan Yanhuang Biological Engineering Co.,Ltd.,Xinxiang 453000,China)
出处
《畜牧与兽医》
CAS
北大核心
2023年第9期60-65,共6页
Animal Husbandry & Veterinary Medicine
基金
河南省现代农业生猪体系中药替抗岗位专家项目(HARS-22-12-G2)
河南省重点研发专项(221111111300)
河南省科技攻关项目(222102110334)。
关键词
响应面法
双黄连口服液醇沉物
乳酸菌
发酵工艺
response surface technology
Shuanghuanglian oral liquid
alcohol sediment
lactic acid bacteria
fermentation process
