摘要
基于Wnt/β-连环蛋白(β-catenin)信号通路探究壮医通痹千斤方对膝关节炎(KOA)大鼠改善作用.通过注射木瓜蛋白酶方法建立KOA大鼠模型,随机分为模型组、治疗组、治疗组+激活剂(氯化锂)组和阳性对照组(塞来昔布胶囊),并以注射等体积的生理盐水作为对照组;收集大鼠外周血及软骨组织,检测各组大鼠血清中白细胞介素(IL)-6和肿瘤坏死因子-α(TNF-α)水平;观察软骨组织显微结构、病理学变化及损伤评分;分析软骨组织中Wnt-3a、β-catenin、runt相关转录因子2(Runx2)mRNA及蛋白表达.与对照组相比,模型组软骨细胞减少且零星分布、膜结构出现边缘空洞或空泡样,IL-6和TNF-α表达水平增加,Wnt-3a、β-catenin和Runx2 mRNA蛋白表达升高以及Mankin评分显著增加(P<0.05).与模型组相比,治疗组和阳性对照组软骨细胞明显增加,排列较为整齐、空泡样结构减少,IL-6和TNF-α表达水平下降、Wnt-3a、β-catenin和Runx2 mRNA蛋白表达降低以及Mankin评分显著降低(P<0.05);与治疗组相比,治疗组+激活剂组细胞排列紊乱,软骨细胞数目减少,空泡样结构增加,IL-6和TNF-α表达水平增加,Wnt-3a、β-catenin和Runx2 m RNA蛋白表达升高以及Mankin评分显著增加(P<0.05).结果表明:壮医通痹千斤方可以改善KOA大鼠损伤,可能与抑制Wnt/β-catenin信号通路有关.
Based on the Wnt/β-catenin signal pathway,the improvement effect of Zhuang medicine Tongbi Qianjin Formula on knee arthritis(KOA)rats is examined.The rat model of KOA was established by injecting papain,and was randomly grouped into model group,treatment group,treatment+activator(lithium chloride)group,positive control group(celecoxib capsule),and the same volume of normal saline was injected as the control group;the peripheral blood and cartilage tissue of rats were collected,and the levels of serum interleukin(IL)-6 and tumor necrosis factor-α(TNF-α)were measured;the microstructure,pathological changes and injury score of cartilage tissue were observed;the mRNA and protein expression of Wnt-3a,β-catenin,runt-related transcription factor 2(Runx2)in cartilage tissue were analyzed.Compared with the control group,the chondrocytes in the model group decreased and scattered,and the membrane structure showed marginal cavities or vacuoles,the IL-6,TNF-α levels,Wnt-3a,β-catenin,Runx2 mRNA and protein expression,and Mankin score were obviously increased(P<0.05);compared with the model group,the number of chondrocytes in the treatment group and the positive control group was obviously increased,the arrangement was more orderly,and the vacuolar structure was reduced,the IL-6,TNF-α levels,Wnt-3a,β-catenin,Runx2 mRNA and protein expression,and Mankin score were obviously decreased(P<0.05);compared with the treatment group,the cells in the treatment group+activator group were arranged in disorder,the number of chondrocytes decreased,and the vacuolar structure increased,the IL-6,TNF-α levels,Wnt-3a,β-catenin,Runx2 mRNA and protein expression,and Mankin score were obviously increased(P<0.05).Zhuang medicine Tongbi Qianjin Formula can improve the injury of KOA rats,which may be related to the inhibition of Wnt/β-catenin signal pathway.
作者
蒋桂江
李凤珍
邹敏
王成龙
龙朝阳
秦祖杰
Jiang Guijiang;Li Fengzhen;Zou Min;Wang Chenglong;Long Chaoyang;Qin Zujie(Department of Rheumatology,Guangzi International Zhuang Medical Hospital&Guangri Intermational Zhuang Medicine Hospital Affiliated to Guangri University of Chinese Medicine,Nanning 530200,China;Diagnosis and Treatment Center of Guangri Zhuang Medicine Rheumatism,Nanming 530200,China;Department of Pediatrics,Guangri Intermational Zhuang Medical Hospital&Guangri International Zhuang Medicine Hospital Affiliated to Guangri University of Chinese Medicine,Namming 530200,China;Ofice of Ethnic Medical,Guangri Intermational Zhuang Medical Hospital&Guangri International Zhuang Medicine Hospital Affiliated to Guangri University of Chinese Medicine,Nanning 530200,China;Characteristic Diagnosis and Treatment Center of National Medicine,Guangri International Zhuang Medical Hospital&Guangri International Zhuang Medicine Hospital Affiliated to Guangri University of Chinese Medicine,Nanming 530200,China;College of Clinical Medicine,Guangri University of Chinese Medicine,Naming 530200,China)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第3期46-51,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家中医药管理局第七批全国老中医药专家学术经验继承工作指导老师及继承人建设项目(国中医药人教函[2022]76号)
国家中医药管理局“十二五”中医药重点学科民族药学(壮药学)(桂中医药函[2021]139号)
广西中医药适宜技术开发与推广项目(GZSY22-46)
广西中医药重点学科(壮医学)建设项目(GZXK-Z-20-60)
广西名中医传承工作室建设项目(桂中医药科教发[2021]6号)。
