摘要
本文以美藤果叶醇提物(Sacha inchi leaf alcohol extract,SILAE)为研究对象,通过构建秀丽隐杆线虫(以下简称线虫)衰老模型,探究了不同质量浓度(5、10、15 mg/mL)SILAE对线虫寿命、运动能力、生殖能力、抗氧化应激能力与体内ROS、脂褐质和丙二醛(MDA)水平的作用效果,以及SILAE对线虫体内酶和非酶抗氧化剂的影响,旨为美藤果叶的进一步研究和应用提供一定的理论参考。结果表明:与空白组相比,5、10 mg/mL的SILAE能显著延长线虫寿命(P<0.05),分别提高了59.3%、30.2%,15 mg/mL SILAE也能延长其寿命但无显著性差异。与此同时,SILAE还能有效提高其生殖能力和运动能力。在氧化应激环境中,SILAE使线虫的H_(2)O_(2)应激寿命分别延长了约2、4、5倍,5、10 mg/mL的SILAE也显著提高了线虫的胡桃醌应激寿命(P<0.05)。SILAE能不同程度地降低线虫体内的ROS和脂褐质(P<0.001),以及MDA(P<0.05)的积累量。此外,15 mg/mL SILAE极显著提高了线虫体内超氧化物歧化酶和过氧化氢酶的活性(P<0.001),谷胱甘肽过氧化酶活性和谷胱甘肽含量分别在5 mg/mL(P<0.05)和10 mg/mL(P<0.01)SILAE组中得到显著提高。综上,SILAE具有抗衰老功效,是具有缓解氧化、延缓衰老的功能食品或替代药物的一种新的可能来源。
In this study,the effects of different mass concentrations(5,10 and 15 mg/mL)of Sacha inchi leaf alcohol extract(SILAE)on Caenorhabditis elegans(C.elegans)longevity,mobility,reproductive capacity,resistance to oxidative stress and levels of ROS,lipofuscin and malondialdehyde(MDA)in vivo,as well as the effects of SILAE on enzymatic and non-enzymatic antioxidants in C.elegans were investigated by constructing a senescence model of C.elegans,with the aim of providing certain theoretical references to the further research and application of Sacha inchi leaf.The results showed that SILAE at 5 and 10 mg/mL significantly prolonged the life span of C.elegans compared with the blank group(P<0.05)by 59.3%and 30.2%,respectively,and 15 mg/mL SILAE also prolonged their life span but without significant differences.Meanwhile,their reproductive and motility abilities were also effectively improved by SILAE.In oxidative stress environments,SILAE prolonged the life span of C.elegans under H_(2)O_(2) stress by approximately 2,4,and 5-fold,respectively,and 5 and 10 mg/mL of SILAE also significantly prolonged the life span of C.elegans under juglone stress(P<0.05).The accumulation of ROS,lipofuscin(P<0.001),and MDA(P<0.05)in C.elegans was reduced to varying degrees by SILAE.In addition,both superoxide dismutase and catalase activities in C.elegans were highly significantly increased by SILAE(P<0.001),while glutathione peroxidase activity and glutathione content were significantly increased by SILAE at 5 mg/mL(P<0.05)and 10 mg/mL(P<0.01),respectively.In conclusion,SILAE has anti-aging property and is a new possible source of functional foods or alternative drugs with oxidative mitigation and aging retardation effects.
作者
赖玉萍
陈颖仪
魏婉娉
安苗青
邹泽斌
杜冰
黎攀
LAI Yuping;CHEN Yingyi;WEI Wanping;AN Miaoqing;ZOU Zebin;DU Bing;LI Pan(College of Food Science,South China Agricultural University,Guangzhou 510642,China)
出处
《食品工业科技》
CAS
北大核心
2023年第21期402-411,共10页
Science and Technology of Food Industry
基金
国家自然科学基金青年基金项目(32202058)
广东省普通高校重点领域专项(生物医药与健康)(2022ZDZX2079)
广东食品药品职业学院校级课题(2021ZR06)。
关键词
美藤果叶
醇提物
秀丽隐杆线虫
氧化衰老
Sacha inchi leaf
alcohol extract
Caenorhabditis elegans
oxidative aging
