摘要
采用傅里叶变换红外光谱结合曲线拟合技术研究存储5年的姜科药材,以探寻姜科药材存储五年的成分变化情况。结果显示,原始红外光谱整体相似,光谱反映出药材的主要物质有挥发油类、黄酮类、糖类、氨基酸等。对谱图差异较大的1300~1450 cm^(-1)进行原始谱图局部放大处理、二阶导数处理,明显看出1384 cm^(-1)、1410 cm^(-1)峰强、峰位差异明显,推断含异丙基和叔丁基化合物的含量及化合物内部结构发生变化。结合吸光度比值法和曲线拟合面积定量分析得出,22种姜科药材的挥发油含量随着储藏时间的变化呈下降趋势,糖类含量呈上升趋势。研究表明,傅里叶变换红外光谱结合曲线拟合可为姜科药材的品质分析提供一种有效手段。
Fourier transform infrared spectroscopy combined with curve fitting technology was used to study the ginger medicinal materials stored for 5 years,in order to explore the composition changes of ginger medicinal materials stored for 5 years.The results showed that the original infrared spectra were similar,and the main substances reflected in the spectra were volatile oils,flavonoids,sugars,amino acids,etc.Local amplification and second derivative processing of original spectra of 1300~1450 cm^(-1)with large differences in spectra showed that 1384cm^(-1)and 1410cm^(-1)had significant differences in peak strength and peak position,and it was inferred that the content and internal structure of compounds containing isopropyl and tert-butyl had changed.Combined with absorbance ratio method and curve fitting area quantitative analysis,the content of volatile oil of 22 species of ginger showed a decreasing trend and the content of carbohydrate showed an increasing trend with the change of storage time.The results showed that Fourier transform infrared spectroscopy combined with curve fitting could provide an effective method for quality analysis of ginger.
作者
刘艳
尹德都
潘宏源
司民真
LIU Yan;YIN Dedu;PAN Hongyuan;SI Minzhen(Yunnan Key Laboratory of universities for Molecular Spectrum,Institute of spectral applied technology,Chuxiong Normal University,Chuxiong 675000,China)
出处
《光散射学报》
2023年第3期275-285,共11页
The Journal of Light Scattering
基金
2021年云南省大学生创新创业训练计划建设项目,项目编号:202111391031。
关键词
红外光谱
姜科
曲线拟合
中药材
Infrared spectrum
ginger
curve fitting
Chinese medicinal materials
