摘要
为提高Zn^(2+)稳定性及生物利用度,以木瓜籽为原料,通过酶水解制备木瓜籽肽(CQSP),经超滤获得5个不同分子质量范围的多肽组分,并制备CQSP-Zn螯合物。考察了蛋白酶种类和多肽分子质量对Zn^(2+)螯合率的影响,运用氨基酸组成分析、红外光谱(FT-IR)、紫外-可见光谱(UV-Vis)、荧光光谱对螯合物结构特征进行表征,并探究了CQSP-Zn在不同pH值和消化阶段的溶解度。研究结果表明:碱性蛋白酶水解的分子质量<1000 u的CQSP对Zn^(2+)螯合率最高,为83.26%。红外光谱、紫外光谱及荧光光谱分析表明:CQSP与Zn^(2+)间产生了相互作用,CQSP中的—NH、CH 2、C O、C—N、—COOH可能参与了对Zn^(2+)的螯合。相对于无机ZnSO 4,CQSP-Zn在pH值2.0~8.0及模拟胃肠道消化过程中具有更高的溶解度,pH值为8.0时,CQSP-Zn的Zn^(2+)溶解度为73.85%,达到ZnSO 4的11.19倍;在pH值为7.5的模拟肠液中,消化第0 min时CQSP-Zn的Zn^(2+)溶解度为65.86%,显著高于ZnSO 4的16.24%,表明CQSP-Zn具有作为Zn^(2+)增补剂的潜力。
In order to improve the stability and bioavailability of Zn^(2+),the Chinese quince seed peptide(CQSP)was prepared by enzymatic hydrolysis using Chinese quince seed as raw material.Five peptide fractions with different molecular weight ranges were obtained by ultrafiltration,and they were applied for preparation of CQSP-Zn chelate.The effects of protease types and peptide molecular weight on the chelation rate of Zn^(2+)were investigated.The amino acids composition analysis,Fourier transform infrared(FT-IR)spectroscopy,ultraviolet-visible(UV-Vis)spectroscopy and fluorescent spectroscopy were used to identify the structural characteristics of the chelate,and the solubility of CQSP-Zn at different pH values as well as digestion stages were also studied.The results indicated that the CQSP with the molecular weight less than 1000 u hydrolyzed by alkaline protease presented the highest Zn^(2+)chelating rate(83.26%).The analysis of FT-IR spectroscopy,UV-Vis spectroscopy and fluorescent spectroscopy revealed the interaction between CQSP and Zn^(2+),and the—NH,CH 2,C O,C—N,—COOH in CQSP might participate in the chelating of Zn^(2+).Compared with the inorganic ZnSO 4,CQSP-Zn showed higher solubility at pH 2.0-8.0 and simulated gastrointestinal digestion procedure.When the pH value was 8.0,the Zn^(2+)solubility of CQSP-Zn was 73.85%,which was 11.19 times higher than that of ZnSO 4.In the simulated intestinal fluid with a pH value of 7.5,the Zn^(2+)solubility of CQSP-Zn was 65.86%at 0 minute of digestion,significantly higher than ZnSO 4(16.24%),demonstrating potential application of CQSP-Zn as Zn^(2+)supplement.
作者
邓叶俊
黄立新
张彩虹
谢普军
DENG Yejun;HUANG Lixin;ZHANG Caihong;XIE Pujun(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China)
出处
《林产化学与工业》
CAS
CSCD
北大核心
2023年第5期73-80,共8页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(32171732)。
关键词
肽
螯合物
模拟消化
生物利用度
peptide
chelate
simulate digestion
bioavailability
