摘要
目的 探讨急性髓系白血病(AML)患者miR-363表达与其临床特征及靶基因的关系。方法 下载TCGA数据库中AML患者的miRNA、RNA表达谱及临床数据,按照miR-363表达水平由高到低排序,将前50%设为高表达组(n=81),后50%设为低表达组(n=81),比较两组患者生存曲线及年龄差异;利用Omisc软件,采用Pearson相关性分析筛选与miR-363表达相关的靶基因。将2021年12月至2022年12月在我院诊治的52例AML患者纳入研究,采用RT-qPCR技术检测患者骨髓miR-363表达水平,按照表达水平由高到低排序,将前50%设为高表达组(n=26),后50%设为低表达组(n=26),比较两组患者初诊白细胞计数、血小板计数、血红蛋白及血浆靶基因水平、年龄、性别及预后分层的差异。结果 TCGA数据库分析显示,miR-363低表达组患者中位生存时间高于miR-363高表达组(822d vs 365d,P<0.05);miR-363高表达组高龄患者比例明显高于低表达组(54.32%vs 45.68%,P<0.05);共筛选出5 404个miR-363相关基因,KIAA1377相关性最高(r=0.62,P<0.05)。miR-363高表达组高龄患者比例、预后不良患者比例、初诊白细胞计数≥50×109/L比例、血浆KIAA1377水平明显高于低表达组(均P<0.05);血小板计数、血红蛋白水平明显低于低表达组(均P<0.05)。结论 miR-363高表达与AML患者生存率降低、高龄、预后不良、初诊白细胞计数升高、血小板计数及血红蛋白水平降低有关,且KIAA1377可能是miR-363的靶基因之一。miR-363可能是AML基因治疗和预后评估的新靶点。
Objective To investigate the relationship between miR-363 expression and clinical characteristics and target genes in patients with acute myeloid leukemia(AML).Methods We downloaded miRNA,RNA expression profiles and clinical data of AML patients from TCGA database,and sorted the expression levels of miR-363 from high to low.We set the first 50%as the high expression group(n=81)and the last 50%as the low expression group(n=81),then compared the survival curves and age between groups;We used Omisc software,Pearson correlation analysis to screen target genes related to miR-363 expression.A total of 52 patients with AML diagnosed and treated in our hospital from January,2018 to December,2019 were included in the study.The expression levels of miR-363 were measured by RT-qPCR and were ranked from high to low:the first 50%were set as the high expression group(n=26),and the last 50%were set as the low expression group(n=26).The newly diagnosed leukocytes count,platelet count,hemoglobin content,plasma target gene and age,gender and prognosis were compared between groups.Results TCGA database analysis showed that the median survival time of patients in the low expression group was higher(822d vs 365d,P<0.05);The proportion of elderly patients in the high expression group was higher(54.32%vs 45.68%,P<0.05);A total of 5404 miR-363-related genes were screened,of which KIAA1377 had the highest correlation(r=0.62,P<0.05).The proportion of elderly patients,poor prognosis patients,newly diagnosed leukocytes≥50×109/L and plasma KIAA1377 content was higher,while the platelet count,hemoglobin content were lower in the high expression group of miR-363 than the low expression group(all P<0.05).Conclusion High expression of miR-363 is associated with decreased survival,advanced age,poor prognosis,increased newly diagnosed leukocytes count,decreased platelet count and hemoglobin content in patients with AML.KIAA1377 could be one of the miR-363 target genes,which may be a new target for AML gene therapy and prognosis evaluation.
作者
张冉
逄瑷博
靖彧
ZHANG Ran;PANG Aibo;JING Yu(Department of Hematology,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Medical School of Chinese PLA,Beijing 100853,China)
出处
《标记免疫分析与临床》
CAS
2023年第8期1402-1406,共5页
Labeled Immunoassays and Clinical Medicine
基金
2019年重大新药创制科技重大专项(编号:2019ZX09201-002)。
