摘要
目的研究甲泼尼龙琥珀酸钠(MP)对大鼠下肢高压喷射伤(HPII)的干预作用。方法将大鼠随机分为对照组、模型组(高压喷射伤模型)、治疗组(尾静脉注射MP 30 mg/kg)。6 h后观察喷射面积、肢体循环情况、HE染色检查血管、神经、肌肉病理变化,计算肝指数,ELISA检验血清TNF-α、IL-1β、IL-6含量。多组间比较采用单因素方差分析。结果与对照组相比,模型组肢体循环障碍,血管、神经和肌肉病理明显断裂损伤,肝指数下降,而血清TNF-α、IL-1β和IL-6含量明显增加(P<0.05),与模型组相比,治疗组上述指标改善明显(P<0.05),差异有统计学意义。结论高压喷射伤后注射MP可以减轻大鼠肢体损害,降低肝脏负荷,改善血清炎性指标变化。
Objective To study the intervention effect of methylprednisolone(MP)on high pressure injection injuries(HPII)of lower limbs of rats.Methods Thirty rats were randomly divided into control group,model group(high-pressure injection injury model)and treatment group(tail vein injection of MP 30 mg/kg).After 6 hours,the jet area and limb circulation were observed,blood vessels,nerve and muscle tissues were examined by HE staining microscopy.Liver index was calculated and serum TNF-α,IL-1βand IL-6 were tested by ELISA.Comparison between multiple groups was performed by One-way Anova.Results Compared with the control group,limb circulation,blood vessels,nerves and muscles were significantly damaged.The liver index decreased while the serum content of TNF-α,IL-1β,and IL-6 all increased significantly(P<0.05).Compared with the model group,the above indicators improved significantly in the treatment group(P<0.05).Conclusions Injection of MP after high-pressure injection injuries can alleviate limb damage,reduce liver load and improve serum inflammatory markers of injured rats.
作者
穆钛飞
史业弘
王成
陈世玖
MU Taifei;SHI Yehong;WANG Cheng;CHEN Shijiu(Department of Plastic and Hand Surgery,the Fifth Affiliated(Zhuhai)Hospital of Zunyi Medical University,Zhuhai 519100,China)
出处
《基础医学与临床》
2023年第11期1680-1684,共5页
Basic and Clinical Medicine
基金
广东省医学科学技术研究基金(A2019322)。
关键词
高压喷射伤
甲泼尼龙琥珀酸钠
动物模型
high pressure injection injuries
methylprednisolone
animal model
