摘要
近年来,海底热液环境中的微生物及其环境适应机制已经成为海洋科学研究的热点。目前,相关的研究主要集中在表层沉积物及微生物的水平分布多样性方面,而对柱状沉积物中微生物垂直分布多样性研究却很少。本文基于西太平洋冲绳海槽南部热液区附近S2站位的柱状沉积物样品,通过对其不同层位的样品进行分离培养和16S rRNA基因高通量测序,揭示了样品中可培养微生物和总体微生物的垂直群落分布特征,同时结合对样品主量元素、微量元素、碳氮含量等指标的评估和冗余分析等统计学方法,讨论了微生物群落结构及其对环境因子的响应。研究发现该位点的柱状沉积物有机质含量较为贫乏,存在两个富含Cu-Zn-Pb的层;各个层位的沉积物中微生物类群均以变形菌为主要类群,同时表层沉积物表现出更高的微生物多样性。此外研究还表明柱状沉积物中有机碳含量与其微生物的群落组成有着更为密切的关系。总之,本研究的结果和获得的菌种资源为进一步深入研究海底热液环境中微生物参与元素地球化学循环的过程提供了一定的基础。
In recent years,microbe and its adaptation mechanism in submarine hydrothermal environment have become the focus of marine science research.At present,relevant researches focus on the horizontal distribution diversity of surface sediments and microorganisms,and only few researches on the vertical distribution diversity of microorganisms in columnar sediments.Based on the columnar sediment samples from the S2 station in the southern hydrothermal area of the Okinawa Trough in the western Pacific Ocean,we revealed the vertical community distribution characteristics of culturable microorganisms and the overall microorganisms in the samples through isolation,culture,and high-throughput sequencing of 16S rRNA gene from the samples at different levels.At the same time,the microbial community structure and its response to environmental factors were discussed by using statistical methods such as the evaluation of major elements,trace elements,carbon and nitrogen contents,and redundancy analysis.Results show that the organic matter content of the core sediment at this site is relatively poor,and Cu-Zn-Pb is rich in two layers;the microbial community of each layer is composed of mainly Proteobacteria,while the surface sediments exhibit higher microbial diversity.Meanwhile,it indicated a closer relationship between the organic carbon content of core sediments and the composition of their microbial communities.This study obtained bacterial strain resources and provided a basis for further research into microbial participation in the geochemical cycle of elements in submarine hydrothermal environment.
作者
邓兴渝
曹文瑞
姜明玉
曾志刚
常凤鸣
宋召军
DENG Xingyu;CAO Wenrui;JIANG Mingyu;ZENG Zhigang;CHANG Fengming;SONG Zhaojun(College of Earth Science and Engineering,Shandong University of Science and Technology,Qingdao 266590,China;Key Laboratory of Marine Geology and Environment,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)
出处
《海洋地质与第四纪地质》
CAS
CSCD
北大核心
2023年第5期198-213,共16页
Marine Geology & Quaternary Geology
基金
山东省自然科学基金面上项目“冲绳海槽唐印热液区周边沉积物中硫氧化菌多样性及其硫代谢途径的比较研究”(ZR2020MD088)
国家自然科学基金面上项目“西太平洋铁锰结核的生物成矿特征及其成矿元素的微生物迁移过程研究”(41976202)
中国科学院海洋地质与环境重点实验室开放基金课题(MGE2022KG4,MGE2022KG6)
国家重点基础研究发展计划(973计划)项目“典型弧后盆地热液活动及其成矿机理”(2013CB429700)
泰山学者工程“海底热液系统、岩浆活动、沉积记录与结核结壳研究”(ts201511061)。
关键词
海底热液
微生物群落结构
柱状沉积物
环境因子
冲绳海槽
seafloor hydrothermal field
microbial community
core sediment
environmental factors
Okinawa Trough
